In the ever-evolving field of molecular biology and genomics, the ability to precisely label and detect oligonucleotides (short synthetic DNA or RNA molecules) is of paramount importance. From gene expression analysis to molecular diagnostics, these tiny molecules serve as powerful tools for unraveling the intricate workings of life at the molecular level. Enter Quasar 670 Amidite, a specialized phosphoramidite reagent that has revolutionized the way we incorporate fluorescent dyes into oligonucleotides.

A Brilliant Red Beacon

Quasar 670 Amidite is a game-changer in the world of oligonucleotide synthesis, allowing researchers to precisely incorporate a brilliant red fluorescent cyanine dye (Quasar 670) into their oligonucleotide sequences. With a maximal emission wavelength around 670 nanometers, this dye offers a vibrant alternative to the commonly used Cyanine-5 (Cy5) dye, expanding the palette of available fluorescent labels.One of the key advantages of Quasar 670 is its exceptional compatibility with black hole quenchers, such as BHQ-2. This unique property enables the creation of highly sensitive fluorescence-based assays and probes, where the dye's fluorescence is efficiently quenched until a specific event triggers its release, resulting in a detectable signal.

Precision and Versatility

The beauty of Quasar 670 Amidite lies in its ability to precisely incorporate the fluorescent dye at the desired position within the oligonucleotide sequence. Unlike traditional labeling methods, which often rely on non-specific or random labeling, this phosphoramidite reagent allows researchers to strategically place the dye at the 5' end of the oligonucleotide, ensuring optimal performance and minimizing potential interference with hybridization or enzymatic reactions.Moreover, the versatility of Quasar 670 Amidite extends beyond end-labeling. By employing specialized synthesis techniques, such as the use of UltraMILD monomers and optimized oxidation conditions, researchers can even incorporate the dye internally within the oligonucleotide sequence, opening up new possibilities for designing advanced molecular probes and sensors.

Handling and Synthesis Considerations

While the power of Quasar 670 Amidite is undeniable, its successful incorporation into oligonucleotides requires careful handling and synthesis considerations. As a light-sensitive reagent, prolonged exposure to light should be avoided to prevent photobleaching and maintain the dye's integrity. Additionally, proper storage and handling techniques are crucial to prevent oxidation, which can compromise the reagent's performance.During the deprotection step, which removes the protective groups from the synthesized oligonucleotide, the use of concentrated ammonium hydroxide is recommended, as methylamine can potentially cause issues with the Quasar 670 dye.

Illuminating the Future

As the field of molecular biology continues to push the boundaries of what is possible, the demand for advanced labeling and detection techniques will only grow. Quasar 670 Amidite represents a significant step forward in this direction, enabling researchers to create highly specific and sensitive oligonucleotide probes and assays.From unraveling the complexities of gene expression to developing cutting-edge molecular diagnostics, the brilliant red beacon of Quasar 670 Amidite is poised to illuminate the path towards a deeper understanding of the molecular world. With its precision, versatility, and compatibility with state-of-the-art quenching technologies, this remarkable reagent is empowering scientists to explore new frontiers and unlock the secrets hidden within the intricate dance of nucleic acids.

High-Quality Fluorescently Labeled Oligonucleotide Synthesis: Choose SBS Genetech

When selecting reagents for fluorescently labeled oligonucleotide synthesis, quality and reliability are paramount. As an industry-leading biotechnology company, SBS Genetech is committed to providing researchers with high-quality fluorescently labeled DNA Synthesis Products. We utilize advanced synthesis technology and rigorous quality control systems to ensure that each batch of products boasts exceptional purity and stability.

Our Advantages Include:

• Strict Quality Control: Each batch undergoes rigorous quality testing, including spectroscopic analysis and mass spectrometry verification, to ensure its fluorescent performance and chemical stability.
• Custom Services: We offer custom synthesis services tailored to customer needs, meeting various experimental requirements, including special modifications, specific sequences, and labels.
• Technical Support: Our expert team provides comprehensive technical support and consultation services to help customers resolve various issues encountered during experiments.

Extensive Application Areas

Our labeled oligonucleotides are widely used in the following research areas:

• Gene Expression Analysis: Highly sensitive qPCR for precise measurement of gene expression levels.
• Pathogen Detection: Rapid and accurate detection of various pathogens, including viruses, bacteria, and fungi.
• Genomic Research: Used in genome sequencing, SNP detection, and mutation analysis, driving advancements in genomics research.
• Molecular Interaction Studies: Utilizing FRET technology to study molecular interactions, revealing dynamic mechanisms of biomolecules.

Welcome to Order

At SBS Genetech, we are dedicated to advancing the frontiers of scientific research. We understand the complexity and rigor of scientific work, and it is our responsibility and mission to provide high-quality reagents and excellent service. If you need high-quality fluorescent labeling reagents, please feel free to contact us. We are committed to providing you with superior products and professional support, helping you achieve greater success in your research endeavors.

Choosing SBS Genetech means choosing excellence and reliability. Let us work together to contribute to the bright future of scientific research.