In the ever-evolving landscape of molecular biology and genomics, the ability to precisely label and detect oligonucleotides (short synthetic DNA or RNA molecules) has become an indispensable tool. From unraveling the intricate mechanisms of gene expression to developing cutting-edge diagnostic assays, these tiny molecules serve as powerful probes, illuminating the intricate dance of life at the molecular level. Among the arsenal of labeling reagents, TET Phosphoramidite (5' Tetrachlorofluorescein-CE Phosphoramidite) stands out as a versatile and powerful ally, enabling researchers to incorporate a brilliant green fluorescent dye into their oligonucleotide sequences.
A Vibrant Green Beacon
TET Phosphoramidite is a specialized phosphoramidite reagent that allows for the site-specific attachment of the tetrachlorofluorescein (TET) dye to the 5' end of an oligonucleotide. This fluorescein derivative boasts an absorption maximum at 519 nanometers and an emission maximum at 535 nanometers, resulting in a vibrant green fluorescence that can be easily detected and quantified.
One of the key advantages of TET-labeled oligonucleotides is their compatibility with dark quenchers, such as DusQ1. This unique property enables the creation of highly sensitive fluorescence-based assays and probes, where the dye's fluorescence is efficiently quenched until a specific event triggers its release, resulting in a detectable signal.
Versatile Applications
The versatility of TET Phosphoramidite is evident in its wide range of applications across various fields of molecular biology and genomics. In the realm of quantitative PCR (qPCR), TET-labeled primers and probes have become indispensable tools for precise gene expression analysis and quantification. Their ability to generate a strong fluorescent signal during the amplification process allows researchers to monitor and quantify target sequences with unparalleled accuracy.
Moreover, TET-labeled oligonucleotides have found widespread use in fragment analysis techniques, such as microsatellite amplification and capillary electrophoresis systems like the ABI PRISM® 310 Genetic Analyzer. By incorporating the TET dye into specific oligonucleotide sequences, researchers can accurately identify and characterize DNA fragments, enabling a deeper understanding of genetic variations and their implications.
Beyond these applications, TET Phosphoramidite has also proven invaluable in the development of molecular beacons, probes, and other fluorescence-based assays and sensors. These innovative tools leverage the unique properties of the TET dye to detect and monitor various molecular interactions, providing insights into biological processes and enabling the development of novel diagnostic and therapeutic approaches.
Precision and Reliability
While the power of TET Phosphoramidite is undeniable, its successful incorporation into oligonucleotides requires careful handling and synthesis considerations. Proper storage and handling techniques are crucial to prevent degradation and maintain the reagent's performance, ensuring consistent and reliable results.
During the oligonucleotide synthesis process, specific coupling conditions, such as extended coupling times, may be required to ensure efficient incorporation of the TET dye. This attention to detail is essential for achieving optimal labeling and maximizing the sensitivity and accuracy of downstream applications.
Illuminating the Future
As the field of molecular biology continues to push the boundaries of what is possible, the demand for advanced labeling and detection techniques will only grow. TET Phosphoramidite represents a significant step forward in this direction, enabling researchers to create highly specific and sensitive oligonucleotide probes and assays.
From unraveling the complexities of gene expression to developing cutting-edge diagnostic tools, the vibrant green beacon of TET Phosphoramidite is poised to illuminate the path towards a deeper understanding of the molecular world. With its versatility, compatibility with state-of-the-art quenching technologies, and proven track record in various applications, this remarkable reagent is empowering scientists to explore new frontiers and unlock the secrets hidden within the intricate dance of nucleic acids.
High-Quality Fluorescently Labeled Oligonucleotide Synthesis: Choose SBS Genetech
When selecting reagents for fluorescently labeled oligonucleotide synthesis, quality and reliability are paramount. As an industry-leading biotechnology company, SBS Genetech is committed to providing researchers with high-quality fluorescently labeled DNA Synthesis Products. We utilize advanced synthesis technology and rigorous quality control systems to ensure that each batch of products boasts exceptional purity and stability.
Our Advantages Include:
- Strict Quality Control: Each batch undergoes rigorous quality testing, including spectroscopic analysis and mass spectrometry verification, to ensure its fluorescent performance and chemical stability.
- Custom Services: We offer custom synthesis services tailored to customer needs, meeting various experimental requirements, including special modifications, specific sequences, and labels.
- Technical Support: Our expert team provides comprehensive technical support and consultation services to help customers resolve various issues encountered during experiments.
Extensive Application Areas
Our labeled oligonucleotides are widely used in the following research areas:
- Gene Expression Analysis: Highly sensitive qPCR for precise measurement of gene expression levels.
- Pathogen Detection: Rapid and accurate detection of various pathogens, including viruses, bacteria, and fungi.
- Genomic Research: Used in genome sequencing, SNP detection, and mutation analysis, driving advancements in genomics research.
- Molecular Interaction Studies: Utilizing FRET technology to study molecular interactions, revealing dynamic mechanisms of biomolecules.
Welcome to Order
At SBS Genetech, we are dedicated to advancing the frontiers of scientific research. We understand the complexity and rigor of scientific work, and it is our responsibility and mission to provide high-quality reagents and excellent service. If you need high-quality fluorescent labeling reagents, please feel free to contact us. We are committed to providing you with superior products and professional support, helping you achieve greater success in your research endeavors.
Choosing SBS Genetech means choosing excellence and reliability. Let us work together to contribute to the bright future of scientific research.