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Cat. No.: ZLIC-20 (for 20 ea)

Zearalenone (ZEN), also known as F-2 toxin, is primarily produced by strains of Fusarium species. Its components are compounds of the 2,4-dihydroxyphenylmethane lactone class produced by Fusarium graminearum and Fusarium culmorum. 

Zearalenone Immunoaffinity Column selectively adsorbs zearalenone from the sample solution, thereby providing highly targeted purification of the sample. The sample solution after column purification can be directly used for HPLC analysis or analyzed by LC-MS/MS after nitrogen blowdown and reconstitution.

Principle

The assay is based on antigen-antibody reaction. Antibodies are immobilized within the column. After sample extraction and filtration, the sample slowly passes through the zearalenone immunoaffinity column. Within the column, the toxin binds to the antibodies. Subsequently, washing removes any unbound substances. Zearalenone is then eluted with methanol and injected into the analytical instrument for detection.

Precautions

• Before use, the immunoaffinity column must be equilibrated to room temperature (22-25°C).
• Store the affinity column at 2-8°C; do not freeze.
• Do not use immunoaffinity columns that have exceeded their expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased according to requirements, but ensure that the volume of the extraction solution is adjusted accordingly.
• Column capacity: 2000 ng. If the content of the toxin to be detected in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample solution should be appropriately reduced for retesting.
• pH: The pH of the sample solution loaded onto the affinity column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Zearalenone can be carcinogenic, so protective gear such as gloves and masks should be worn during operation.
• It is advisable to soak used containers and standard solution solutions overnight in a sodium hypochlorite solution (5% V/V).
• Consistency between solvents and mobile phases during machine detection can eliminate the solvent effect.

Related:

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• Protein G Immunoaffinity Column
• T-2 Toxin Immunoaffinity Column
• T-2/HT-2 Toxin Immunoaffinity Column
• Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column
• Fungal Toxin 3-in-1 (AFT Total, ZEN, DON) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFT, OTA) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column

Only for research and not intended for treatment of humans or animals

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