All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: WBMR-50 (for 50T)

Cat. No.: WBMR-200 (for 200T)

Description

In recent years, with the extensive research on RNA interference and regulatory small RNAs, there has been an urgent need for a kit that can efficiently extract RNA of approximately 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods such as silica gel membranes cannot effectively adsorb and recover these molecules. Phenol/guanidine extraction and ethanol precipitation also do not effectively precipitate and recover small molecule RNAs. Blood samples, in particular, are even more challenging to extract due to their unique characteristics.

This kit uses a unique lysis solution to quickly and directly lyse whole blood (liquid samples) and inactivate cellular RNases. It employs a robust organic extraction to remove proteins and DNA. RNA, including small molecule RNA, is adsorbed onto a special silica-based membrane in the centrifuge column. This is followed by a series of rapid wash-centrifugation steps using a washing solution, which further removes cellular metabolites, proteins, and other impurities. Finally, the RNA is eluted from the silica-based membrane using a low-salt elution solution.

Features

• The silica-based membranes inside the centrifuge adsorption columns are all custom-made by a world-renowned company. The adsorption capacity varies very little between columns, ensuring good repeatability. This overcomes the inconsistency in membrane quality found in ordinary kits.
• There is no need for steps like ethanol precipitation, which can easily lead to the loss of small molecule RNA.
• The kit features a unique lysis solution formula that can directly lyse whole blood without the need for initial lysis to remove red blood cells.
• Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9 to 2.0, and virtually no DNA residue, making it suitable for RNAi, RT-PCR, Northern-blot, and various other experiments.