All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: WBE-100 (for 100ml)

Cat. No.: WBE-500 (for 500ml)

Description

Western Blot Enhancer is a reagent that enhances the specific binding between antigen and antibody during Western blot detection by directly diluting it with primary or secondary antibodies. It can significantly increase the sensitivity of Western blot detection by several-fold to more than ten-fold.

This product is derived from large-scale and fine screening to obtain high-molecular compounds that likely interact specifically with antigen-antibody complexes through adhesion, entanglement, or binding. It also includes complementary blocking reagents and detergents to reduce background, effectively addressing common issues encountered in Western blotting, such as low sensitivity and high background. It greatly improves the signal-to-noise ratio. This product can also be used for enhancing immunofluorescence, immunohistochemistry, dot blot, ELISA, and other immunodetection methods involving antigen-antibody interactions. It can also be attempted for enhancing other protein-protein interaction assays.

Western blot, also known as Western blotting or immunoblotting, often abbreviated as WB, is one of the main methods for protein expression analysis using antibodies. The typical steps involve SDS-PAGE electrophoresis, membrane transfer, blocking, primary antibody incubation, secondary antibody incubation, and detection using labeled secondary antibodies.

During Western blot experiments, common issues encountered include weak signal bands due to low abundance of the target protein or low antibody potency, high background due to poor blocking, and non-specific bands caused by poor antibody specificity. Resolving such issues often requires extensive optimization, especially when changing antibodies or increasing antibody dosage, which can increase experimental costs.

Features

• This product greatly enhances the signal intensity of target bands: By promoting specific binding between antigen and antibody, the signal intensity generated by this product can be several-fold to over ten-fold higher than traditional methods. It can improve the success rate of detecting low-abundance proteins or reduce the amount of protein samples required.
• This product provides better detection results: It effectively reduces background noise and enhances the signal intensity of target bands, enabling more desirable Western blotting results.
• This product improves antibody specificity: It contains highly effective blocking reagents and detergent combinations that reduce non-specific binding, thereby enhancing antibody specificity and reducing non-specific band signals, resulting in improved Western blotting results.
• This product saves on antibody usage: Compared to traditional methods, this product significantly enhances the detection signal, allowing for a reduction in the amount of primary and secondary antibodies used, thereby saving on experimental costs. Additionally, the diluted primary or secondary antibodies prepared with this product can be stored at 4°C and reused multiple times for Western blotting, facilitating antibody conservation.
• This product is convenient to use: It is a ready-to-use solution that can be directly diluted with primary and secondary antibodies. There is no need to distinguish between dilutions for primary or secondary antibodies, and it does not add any additional experimental steps or alter the standard Western blotting protocol. For challenging target proteins, it saves time and effort in optimizing protein detection.
• This product is compatible with blotting membranes: It is compatible with both PVDF (Polyvinylidene Fluoride) membranes and NC (Nitrocellulose) membranes.
• This product is compatible with detection substrates: The components of this product do not affect the enzyme activity of various labeled antibodies. It is compatible with horseradish peroxidase (HRP), alkaline phosphatase (AP), and biotin-labeled secondary antibodies, making it suitable for chemiluminescence (ECL), colorimetric, and fluorescence-based detection methods.
• This product has a wide range of applications: It is particularly suitable for Western blotting but can also be used in immunofluorescence, immunohistochemistry, dot blot, ELISA, and other immunodetection methods involving antigen-antibody interactions.

Precautions

• The diluted primary or secondary antibodies prepared with this product can be stored at 4°C after use and can be reused for Western blotting. However, if the detection results show a decrease in performance, turbidity, or precipitation, it is advisable to discard the antibodies.
• To ensure that the diluted primary or secondary antibodies can be reused multiple times, it is recommended to store them at 4°C immediately after antibody incubation for subsequent reuse.
• This product is recommended for diluting and preparing primary or secondary antibodies and is not intended to replace membrane blocking in Western blotting or for antigen or antibody coating in ELISA detection.
• This product has been optimized and has good blocking activity, so there is no need to add other blocking reagents such as BSA, skim milk, or casein when diluting primary or secondary antibodies. Adding additional blocking reagents may have negative effects.
• Due to the variability of target proteins and antibodies, the enhancement effect on the signal intensity of certain target proteins and corresponding antibodies may vary.
• During the actual use of this product, if the signal of the target band is very strong, the signal of non-specific bands may also be enhanced accordingly. If the non-specific bands are too close to the target protein band and affect the detection results, you can try reducing the protein loading amount, lowering the concentration of primary and secondary antibodies, or shortening the exposure time.
• This product is intended for scientific research purposes by professionals only and should not be used for clinical diagnosis or treatment, food, or drugs. It should not be stored in regular residential areas.
• For your safety and health, please wear lab coats and disposable gloves when handling this product.

Storage

Store at 4°C, valid for one year. For long-term storage without use, store at -20°C.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory