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Cat. No.: VB12IC-20 (for 20 ea)

VB12, also known as cyanocobalamin, antipernicious anemia vitamin, etc., is an important B vitamin. Inadequate intake may lead to diseases such as pernicious anemia and mental fatigue.

Vitamin B12 (VB12) Immunoaffinity Column selectively adsorbs VB12 from the sample solution, thereby providing targeted purification of VB12 in complex matrices. The purified sample after column purification can be directly used for HPLC or LC-MS analysis.

The combination of the affinity column with HPLC or LC-MS achieves rapid determination to improve signal-to-noise ratio and enhance the accuracy of the detection method.

Principle

The assay is based on antigen-antibody reaction. Antibodies are immobilized within the column. After sample extraction, centrifugation, and filtration, the sample slowly passes through the VB12 immunoaffinity column. Within the column, VB12 binds to the antibodies. Subsequently, washing removes any unbound impurities. VB12 is then eluted with methanol, dried with nitrogen, and reconstituted for injection into the analytical instrument for detection.

Precautions

• Before use, the immunoaffinity column must be equilibrated to room temperature (22-25°C).
• Store the affinity column at 2-8°C; do not freeze.
• Do not use immunoaffinity columns that have exceeded their expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased according to requirements, but ensure that the volume of the extraction solution is adjusted accordingly.
• Column capacity: 1000 ng. If the content of the VB12 to be detected in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample solution should be appropriately reduced for retesting.
• Gravity or gas-controlled flow rates between 1.5-2 mL/min should be used when passing the column.
• Consistency between solvents and mobile phases during machine detection can eliminate the solvent effect.
• Potassium cyanide is toxic. When handling, wear protective clothing, long-cuffed rubber gloves, goggles, masks, and a hat. Potassium cyanide releases highly toxic hydrogen cyanide gas when in contact with acid. Sodium hypochlorite solution can be added to the waste solution after the reaction, and then discharged. All contaminated containers and gloves should be soaked in a 4% sodium hypochlorite solution for disposal. It is recommended to add some sodium hydroxide to the solution to ensure an alkaline environment. After 24 hours, use cyanide test paper to check.

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Only for research and not intended for treatment of humans or animals

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