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Cat. No.: UAR-10 (for 10KU)

Cat. No.: UAR-100 (for 100KU)

Description

CAS：9002-12-4

Creatinine Amidohydrolase is useful for the enzymatic determination of creatinine when coupled with creatine amidohydrolase and sarcosine oxidase.

Specification

• Appearance: White amorphous powder, lyophilized 
• Protein purity: ≥90% (SDS-PAGE) 
• Activity: ≥20 U/mg solid
• Catalase: ≤0.03%
• NADH/NADPH oxidase: ≤0.01%

Properties

• EC number: 1.7.3.3 (Recombinant from microorganism)
• Molecular weight: 40 kDa (SDS-PAGE)
• Isoelectric point: 5.1 
• Michaelis constants: 2.1×10^-5 M (Urate)
• Inhibitors: Fe3+, Co2+, Cu2+, Mn2+, Zn2+ 
• Optimum pH: 8.5
• Optimum temperature: 37 °C
• pH stability: pH 5.5-10 .0 (25 °C, 16 h)
• Thermal stability: 60 °C (pH 8.5, 30 min) 
• Stabilizer: Borate, EDTA, and nonionic surfactant

Storage

Store at -25 ～-15°C for up to 12 months. Avoid repeated freezing and thawing.