Cat. No.: USTTH-100 (for 100U)

Cat. No.: USTTH-1k (for 1000U)

Description

Ultra-sensitive Tth DNA Polymerase is a recombinant DNA polymerase engineered based on TtH DNA polymerase and Taq DNA polymerase. It exhibits unparalleled amplification efficiency and RT-PCR efficiency. High-speed and efficient cDNA synthesis can be achieved under Mg2+ conditions.

Features

• Wide Applicability: It is widely applicable in various fields such as Fast-PCR, Fast-RT-PCR, and Fast One-Step RT-PCR.

• Rapid DNA and RNA Detection: It possesses rapid DNA and RNA detection capabilities, enabling fast detection of DNA and RNA under Mg2+ conditions.

• Ultra-sensitive: Ultra-sensitive, with sensitivity 500-1000 times higher than Taq.

• High-Speed Polymerase: High-speed polymerase with an extension rate of >200 bp/second, suitable for Fast-qPCR (POCT-qPCR).

• Efficient Reverse Transcription: Efficient reverse transcription, with approximately 1000 bp RNA reverse transcription achievable in 2 minutes at 65°C, which is over 100 times more efficient than MMLV.

• Reversible Hotstart: Reversible Hotstart, utilizing L-DNA adapter (Aptamer) modification, with no enzyme activity below 60°C.

• Multiplex Fluorescence Quantitative PCR and RT-PCR: Suitable for multiplex fluorescence quantitative PCR and RT-PCR.

• Lyophilization: This product is glycerol-free and can be used for preparing freeze-drying systems.

Quality Conrol

• Deoxyribonuclease Endonuclease: <1%.

• Deoxyribonuclease Exonuclease: <1%.

• Ribonuclease Exonuclease Detection: <1%.

• Yield of cDNA Product: Not exceeding 50 IU.

• cDNA Length Detection: >10 kb.

• Direct qRT-PCR: Not exceeding 50 IU.

Storage

This product should be stored under light-protected conditions at -25 to -15°C, transported on dry ice, and has a shelf life of 2 years.

Related: Ultra-sensitive qRT-PCR Lyophilized Microbeads

Only for research and not intended for treatment of humans or animals

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory