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Cat. No.: USRPLyo-48 (for 48T)

Cat. No.: USRPLyo-480 (for 480T)

Description

Ultra-sensitive qRT-PCR Lyophilized Microbeads contain recombinant Ultra-sensitive Tth DNA Polymerase with adapter modification for amplification, dUTP, UDG, Mg²⁺, dNTPs, buffer salts, lyophilization protectants (Poly A and T.E.T), etc. When used, simply add primers and templates for PCR amplification; the system has been optimized with a special formula to meet various PCR amplification needs. The incorporation of the dUTP and UDG system effectively eliminates PCR aerosol contamination.

This product exhibits unparalleled amplification efficiency and RT-PCR efficiency. It achieves rapid and efficient cDNA synthesis under Mg2+ conditions.

Features

• This product is widely applicable in various Fast-PCR, Fast-RT-PCR, and Fast One-Step RT-PCR fields.
• This product possesses rapid DNA and RNA detection capabilities, allowing for swift detection under Mg2+ conditions.
• Ultra-sensitive, with sensitivity 500-1000 times that of Taq.
• High-speed polymerase with an extension rate >200bp/second, suitable for Fast-qPCR (POCT-qPCR).
• Efficient reverse transcription, achieving a reverse transcription rate of around 1000bp in 2 minutes at 65°C, with efficiency over 100 times that of MMLV.
• Reversible Hotstart, utilizing L-DNA adapter (Aptamer) modification, rendering the enzyme inactive below 60°C.
• Applicable for multiplex fluorescence quantitative PCR and RT-PCR.

Applications

• The reaction system is 25µL/RXN.
• Suitable for TaqMan probe method and Sybrgreen dye method for quantitative RT-PCR with fluorescence.
• Applicable to multiplex RT-PCR and multiplex fluorescence quantitative RT-PCR.
• Suitable for rapid reverse transcription: <2 minutes.
• Suitable for high-temperature reverse transcription: 55~60 degrees.

Storage