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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: TTRHII-250 (for 250U)

Cat. No.: TTRHIII-1250 (for 1250U)

Description

Tth Thermostable RNase HII is an endoribonuclease that specifically cleaves the phosphodiester bonds at the 5' end of RNA bases in a DNA double-strand, resulting in a nick and producing a 5' phosphate and 3' hydroxyl end. RNase HII also cleaves the RNA portion along the Okazaki fragment at multiple sites (i.e., RNase H activity). This enzyme does not hydrolyze pure DNA strands or single-stranded RNA. Our RNase HII is recombinantly expressed and purified through multiple steps.

Activity Definition

The enzyme quantity required to cleave 100 pmol of double-stranded DNA substrate containing a single ribonucleotide at 55°C in 1× AM Buffer E within 30 minutes.

Activity Assay Conditions

AM Buffer E, incubation at 55°C.

Features

• Heat-resistant enzyme with activity in the range of 40-75°C.
• Possesses RNase H activity simultaneously.

Applications

• Cleaving double-stranded DNA containing ribonucleotides.
• Generating double-strand breaks at sites of inserted ribonucleotides when used in conjunction with T7 Endo I.
• Degradation of the RNA portion of Okazaki fragments.

Storage

Store at -20°C.

Instruction: Protocol

Related:

• RNase H
• Thermostable RNase H
• Tth Thermostable RNase H
• Tth Thermostable RNase HII
• Extreme Thermostable RNase HII
• RNase HIII

Only for research and not intended for treatment of humans or animals

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