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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: THMS-100 (for 100 μg)

Cat. No.: THMS-500 (for 500 μg)

Description

DNA mismatch repair system (MMR) is mainly composed of three proteins, including MutS, MutL, and MutH. Among them, MutS is responsible for recognizing and binding to mismatched or unbound sites. Subsequently, MutL protein forms a complex with MutS-DNA to enhance its stability, activate the endonuclease activity of MutH, and cooperate with helicase and single-strand binding protein to remove the mismatched sequence. Tth MutL protein is a DNA mismatch repair protein isolated from Thermus thermophilus. It has DNA-dependent ATPase activity in the optimal temperature range of 65-75°C. The Tth MutS protein recognizes the loop structure formed by mismatched bases and inserted bases in DNA replication, and it tightly binds to the loop structure to prevent the extension of polymerase. Therefore, it can be used in the mismatch PCR reactions (ARMS-PCR) and gene chips. In addition, it can prevent the occurrence of mismatches in gene synthesis and reduce the rate of gene mutation.

Applications

• Heat-stable MutS, suitable for mismatch DNA polymerase reactions at high temperatures.
• Removal of mismatched DNA from PCR products.
• Mismatch DNA detection/SNP detection.
• Mismatch DNA enrichment experiments, removing wild-type DNA.

Storage

The minimum shelf life is 2 years at -20°C.