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Cat. No.: TDG2P-50 (for 50 μg)

Cat. No.: TDG2P-250 (for 250 μg)

Description

TtDnaG2 protein is derived from Thermoanaerobacter tengcongensis and has a DNA-dependent RNA polymerase activity initiator (Primase). In DNA replication, it can synthesize RNA primers, and then guide the synthesis of leading strand DNA. Compared with the wild-type TtDnaG, the modified TtDnaG2 protein removes its sequence-specific recognition characteristics, making the protein have no preference for templates in the process of RNA Primer synthesis. The protein is active at 37~85°C, and the optimal reaction temperature is 70°C. It is reported that it has the ability to synthesize RNA primers of more than 100 nt.

Components

50 μg

TtDnaG2 Primase (1 μg/μl):    50 μl

Storage

The minimum shelf life is 3 years at -20°C.

Note

• Storage Buffer: 20mM Tris-HCl (pH7.5), 200mM NaCl, 5mM DTT, 50% glycerol.