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Cat. No.: TEZF-1 (for 1ml)

Cat. No.: TEZF-10 (for 10ml)

Cat. No.: TEZF-100 (for 100ml)

Description

TransEZ293F™ Transfection Reagent is an extremely efficient novel transfection reagent based on cationic liposomes. It is specifically designed for transfecting suspension-cultured HEK293 cells, such as Freestyle™ 293-F, for plasmid transfection and large-scale recombinant protein expression.

The transfection efficiency of TransEZ293F™ Transfection Reagent for Freestyle™ 293-F cells is very high (approximately 85%). It has minimal cell toxicity, good reproducibility, easy operation, and does not require changing the culture medium after transfection.

TransEZ293F™ Transfection Reagent is also suitable for transfecting adherent 293 series cells. However, for transfecting adherent 293 series cells, it is recommended to use the more cost-effective TransEZ293™ reagent.

The presence of serum during transfection with TransEZ293F™ Transfection Reagent does not affect transfection efficiency. This eliminates the need to remove serum during transfection, thereby reducing potential damage to cells caused by the removal of serum, a common step when using other cationic liposome-based reagents.

TransEZ293F™ Transfection Reagent exhibits minimal toxicity and does not require changing the culture medium after transfection. This ensures both optimal cell growth conditions for high-level expression of the target protein and reduces the number of steps involved in the process, saving on culture medium.

After transfection of Freestyle™ 293-F cells with TransEZ293F™ Transfection Reagent, the desired protein expression from the transfected plasmid is typically achieved within 48-72 hours.

The transfection efficiency of TransEZ293F™ Transfection Reagent can be rapidly assessed using plasmids expressing fluorescent proteins such as EGFP.

Each milliliter of TransEZ293F™ Transfection Reagent can transfect 500 milliliters of suspended culture of 293-F cells.

Storage

Store at 4°C. For long-term storage, it can be stored at -20°C. Precipitation may occur after storage at -20°C. To completely dissolve the precipitate, incubate at 37°C for 30 minutes before using for cell transfection.

Precaution

• Using high-purity DNA contributes to achieving higher transfection efficiency.
• After storage at -20°C or lower temperatures, this product may freeze and form precipitate upon dissolution. To completely dissolve the precipitate, incubate at 37°C for 30 minutes before using for cell transfection. This will not affect the performance. Upon receipt, the product may show precipitation after dissolution due to low-temperature transport, such as dry ice. Incubating at 37°C for 30 minutes to ensure complete dissolution will allow normal usage. If a small amount of precipitation remains after the 37°C incubation, it can be gently mixed and used directly. Testing has shown that this will not affect performance.
• Cells must be in a good growth state before transfection.
• Prepare serum-free Freestyle™ 293-F cell culture medium without antibiotics.
• TransEZ293F™ transfection reagent should not be vortexed or centrifuged. Gently tapping with a pipette or slow shaking can be used for mixing.
• After use, immediately close the lid of TransEZ293F™ transfection reagent to avoid prolonged exposure to air, which can affect transfection efficiency.
• This product is harmful to humans. Handle with care, and take necessary precautions to prevent direct contact or inhalation.
• This product is intended for scientific research by professionals only. It should not be used for clinical diagnosis or treatment, or for food or drugs. Do not store it in a regular household setting.
• For your safety and health, wear appropriate lab attire and disposable gloves when handling.

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SBS Genetech is recognized as one of the global major leading industry players in Transfection Reagent by third-party market researchers. For more details, please visit Global Transfection Reagent and Equipment Market – Industry Trends and Forecast to 2030.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory