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Thermostable Tte UvrD Helicase
$200.00 - $1,400.00
$1,750.00
Thermostable Tte UvrD Helicase is derived from thermotolerant bacteria Thermoanaerobacter tengcongensis UvrD gene. The helicase has the ability to unwind DNA from 3' end to 5' end under heat-stable conditions. In thermophilic Helicase-Dependent Amplification (tHDA), the enzyme can assist the polymerase in unwinding and complete isothermal amplification. The enzyme can withstand 65°C and can be used for isothermal amplification independent of the single-stranded binding protein.
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Cat. No.: TTUD-2 (for 2μg)
Cat. No.: TTUD-50 (for 50μg)
Applications
Unwinds double-stranded DNA in isothermal amplification.
Storage
The minimum shelf life is 3 years at -20°C.
Note
- 3mM ATP and 2mM Mg2+ need to be added to the reaction solution.
- Research showed that tHDA reaction can be performed well with 4ng/μl of Tte UvrD Helicase, which does not depend on Tte-MutL single-stranded binding protein.
Only for research and not intended for treatment of humans or animals
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