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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

We also offer DNA-Free TGI-RTase Reverse Transcriptase, which has undergone a rigorous E. coli DNA removal process.

Cat. No.: TGIRT-500 (for 500pmol) 

Cat. No.: TGIRT-10k (for 10nmol) 

Description

TGI-RTase has reverse transcriptase activity that depends on DNA/RNA hybrid chain and can rapidly synthesize cDNA. This enzyme has strong strand switching activity. Therefore, the enzyme can be used for synchronous reverse transcription and ligation of nucleic acid molecules such as tRNA, ncRNA, and miRNA (TGIRT template-switching reaction, for TGIRT-Seq). TGI-RTase has strong extensibility, so it is easier to obtain full-length cDNA. In addition, the strong switch activity of this enzyme makes it possible to use the 5' end cap structure of RNA for SMART Library Building (SMART-Seq). The reaction temperature of the enzyme is 37-60°C, and the optimal reaction temperature is 50°C.

Components

• TGI-RTase Reverse Transcriptase (10 pmol/μl): 50 μl
• 5×TGI-RTase Buffer: 0.5 ml
• 100mM DTT: 100 μl
• 10×Duplex Buffer: 0.5 ml

Features

• Higher thermal stability, persistence, and fidelity than retroviral reverse transcriptase allow full-length end-to-end cDNA synthesis from highly structured or strictly modified RNA.
• New end-to-end template-switching activity, which can connect RNA-seq or PCR linkers during reverse transcription, and no longer requires a separate RNA 3'- linker connecting step
• No RNA ligase is required, and the requirement for template quantity is low, which simplifies the steps of RNA-seq library construction and makes it more efficient.

Applications

• Strand-specific transcriptome analysis
• Whole-cell or cell-free transcriptome sequencing
• Noncoding RNA transcriptome sequencing
• RIP-seq, HITS-CLIP, irCLIP, and CRAC
• Identification of RNA base modifications by high throughput sequencing
• RNA structure mapping
• Synthesis of long cDNA
• Genome-wide or targeted RNA structure analysis

Storage

The minimum shelf life is 2 years at -20°C and 3 months at 4°C.

Inactivation

After the reaction, add 1 μl 5M NaOH and heat at 95°C for 2 min to inactivate the enzyme. After inactivation, add 5M HCl to neutralize the high alkaline conditions.

Instruction: Protocol

Related: 

• Mutant M-MLV Reverse Transcriptase
• Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• TGI-RTase Reverse Transcriptase
• Warm Activated Reverse Transcriptase

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory