TBS-T Quick-Dissolve Pellets (100×1L)
$210.00
$350.00
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Cat. No.: TBSTQD-100 (for 100×1L)
Description
TBS-T is a white to off-white quick-dissolve pouch. Each pouch of TBS-T quick-dissolve pellets can be used to prepare 1 L of 1× TBS-T buffer, making it easy and convenient to use. The primary components of TBS-T include sodium chloride, potassium chloride, Tris buffer salt, and Tween-20. In 1× TBS-T buffer, the concentration of sodium chloride is 137 mM, potassium chloride is 2.7 mM, Tris buffer salt is 25 mM, and the content of Tween-20 is 0.05%.
TBS-T is a widely used buffer in biology and is primarily used in immunohistochemistry, in situ hybridization, enzyme-linked immunosorbent assays (ELISA), and immunoblotting experiments. It is commonly used as a washing buffer and for antibody dilution and blocking solutions.
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Components
TBS-T: 100 Pouches (100×1L)
Protocol
- Measure approximately 600 ml of distilled water and add it to a beaker. Place a magnetic stir bar inside the beaker.
- Position the beaker on a magnetic stirrer and slowly add the entire contents of one pouch of TBS-T quick-dissolve pellets. Stir the solution until it is completely dissolved.
- Add distilled water to the TBS-T solution from step 2, bringing it to a final volume of 1 L, making it 1× concentration.
Storage
Room temperature.
Only for research and not intended for treatment of humans or animals
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