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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: TADL-2k (for 2KU)

Cat. No.: TADL-10k (for 10KU)

Description

Taq DNA Ligase catalyzes the formation of phosphodiester bonds between the 5'-phosphate end and the 3'-hydroxyl end of adjacent DNA strands. This DNA ligation reaction occurs only when two oligonucleotide chains are perfectly complementary to the target DNA and there are no gaps between the two oligonucleotide chains. It is not active on single-stranded DNA or RNA or on blunt-ended DNA. Therefore, it can be used to detect single-base mutations. Taq DNA Ligase utilizes NAD+ as a cofactor. It exhibits activity within the temperature range of 37-75°C. Its high thermal stability allows for ligation under high-stringency hybridization conditions and precise SNP detection with high specificity. Our Taq DNA Ligase is recombinantly expressed and prepared through multi-step purification of the recombinant protein.

Activity Definition

One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 μg of λ DNA fragments (with 12 bp sticky ends) digested with BstEII within 15 minutes under the reaction conditions of 1× Taq DNA Ligase Buffer at 45°C.

Activity Assay Conditions

20 mM Tris-HCl, 25 mM KAc, 10 mM Mg(Ac)2, 10 mM DTT, 1 mM NAD, and 0.1% Triton X-100 (pH 7.6 @ 25°C), incubation at 45°C.

Features

• Excellent thermal stability.
• Suitable for the Gibson assembly method.
• Repair of dsDNA nicks.
• Can be used in Ligase Chain Reaction (LCR), Ligase Detection Reaction, and detection of allele-specific genes.

Storage

Store at -20°C for up to 2 years, avoiding repeated freeze-thaw cycles.

DNA Ligase Products:

• T4 DNA Ligase
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032