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Cat. No.: T4DLGF-50k (for 40KU)

Cat. No.: T4DLGF-500k (for 500KU)

Product Description

T4 DNA Ligase (glycerol-free) is an enzyme that catalyzes the formation of a phosphodiester bond between the 5’-P end and the 3’-OH end of double-stranded DNA or RNA with either sticky or blunt ends. Additionally, T4 DNA Ligase can repair single-strand nicks in double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids, but it is inactive on single-stranded nucleic acids. ATP is required as a cofactor for the ligation reaction.

Our T4 DNA Ligase (glycerol-free) is a recombinant protein expressed and purified through multiple steps, and it is glycerol-free.

Activity Definition

One unit is defined as the amount of enzyme required to ligate 50% of the HindIII-digested λ DNA fragments [5’ end concentration of 0.12 μM (300 μg/ml), total DNA amount 6 μg] in 20 μl of 1×T4 DNA Ligase Reaction Buffer at 16°C for 30 minutes.

Activity Assay Conditions

50 mM Tris-HCl (pH 7.5 @ 25°C), 1 mM ATP, 10 mM MgCl2, 10 mM DTT, incubated at 16°C. The recommended DNA 5’ end concentration is 0.1-1.0 μM.

Features

• Sticky end ligation can be completed in just 1 minute.
• Heat inactivation: 65°C for 10 minutes.

Applications

• Restriction enzyme cloning
• Efficient ligation of sticky and blunt ends
• T/A cloning
• Construction of high-abundance clone libraries
• RNA and DNA ligation
• Repair of single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrids

Storage

Store at -20°C.

DNA Ligase Products:

• T4 DNA Ligase
• T4 DNA Ligase (glycerol-free)
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory