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Cat. No.: SGSP1-5 (for 5×1ml)

Cat. No.: SGSP1-100 (for 100×1ml)

Description

SuperGold™ 1×sPfu MasterMix contains SuperGold™ sPfu DNA Polymerase (sPfu), dNTPs, dyes, and buffers required for PCR reactions. It is a ready-to-use PCR amplification reagent. You only need to add primers and DNA templates to perform PCR amplification. The total volume of primers and template added can be varied between 1 and 10μl, which provides strong adjustability. The mastermix can save experimental time and avoid contamination caused by multiple additions of PCR reagents. The mastermix already contains dyes, so the sample can be directly loaded for the electrophoresis after the amplification. The amplified product has blunt ends.

SuperGold™ sPfu DNA Polymerase has extremely high fidelity, which is 69 times as high as Taq DNA polymerase. Extremely high fidelity makes it possible to clone ultra-long fragments. And simple templates such as plasmids and lambda DNA can be amplified by SuperGold™ High Fidelity PCR MasterMix with an effective length of 40 kb, genomes can be amplified with an effective length of 20 kb, and difficult fragments such as cDNA can be amplified with an effective length of 10 kb. In addition, the extension rate of SuperGold™ High Fidelity DNA polymerase can reach as fast as 15 sec/kb, which is four times as high as Taq DNA polymerase.

This product can be applied to perform high-fidelity PCR amplification, colony PCR, blunt-end PCR product amplification, and RT-PCR.

Features

• High fidelity: ~ 69 times as high as Taq DNA polymerase 
• High extension rate: ~ 4 times as high as Taq DNA polymerase
• Ultra-long fragments: >40 kb for simple templates (plasmids and lambda DNA), >20 kb for genomes, and >10 kb for cDNA 

Storage

The minimum shelf life is 1 year at -20°C.

Instruction: Protocol

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory