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Cat. No.: SMB-1 (for 1ml)

Cat. No.: SMB-5 (for 5ml)

Description

Streptavidin Magnetic Beads, also known as Streptavidin beads or SA beads, are formed by covalently coupling high-quality streptavidin (SA) with superparamagnetic nano-sized beads. These beads can rapidly, efficiently, sensitively, and specifically bind to molecules labeled with biotin, such as antibodies, nucleic acids, proteins, peptides, lectins, and other biomolecules. They are primarily used for the separation and purification of biotin-labeled nucleic acids, antibodies, proteins, or related complexes. They are commonly employed in immunoprecipitation (IP), cell sorting, DNA-protein interaction studies, and other applications.

Streptavidin has a molecular weight of 55 kDa and exhibits high specificity in binding to biotin. The affinity constant (Kd) between streptavidin and biotin is approximately 10-15 M. Streptavidin is a tetrameric protein capable of simultaneously binding four biotin molecules. Streptavidin, also known as "liánméi qīnhé sù" in Chinese, is purified from Streptomyces avidinii and shares a high degree of structural similarity and affinity for biotin with avidin derived from chicken egg whites. However, unlike avidin, streptavidin is non-glycosylated and lacks significant charge. Avidin has a pI of approximately 10.5, rendering it alkaline under neutral pH conditions. Due to the lack of charge under neutral pH conditions, streptavidin exhibits significantly lower nonspecific binding compared to avidin, resulting in reduced nonspecific background in detection. Consequently, streptavidin is commonly used as a substitute for avidin in biotin detection.

Streptavidin magnetic beads find wide applications in the field of biomedicine. They can specifically bind to biotin-labeled antigens or antibodies and serve as carriers for immunoprecipitation, cell sorting, ELISA, and other reactions. They can also be used to isolate specific nucleic acid-protein complexes from cell or tissue extracts by binding to biotin-labeled DNA or RNA fragments, facilitating studies on protein-nucleic acid interactions. Furthermore, they can be employed in DNA-RNA hybridization experiments, mRNA isolation, purification of single-stranded biotin-labeled DNA oligonucleotides, and separation of biotin-labeled PCR products.

Only for research and not intended for treatment of humans or animals

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