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Description

We introduce a specific cell culture serum. By adding this product to the basic culture medium of the relevant cells, along with dual antibodies and necessary additives, it can be utilized for the cultivation of the associated cells.

Product List

• 22Rv1 Cell Culture Serum
• 293 Cell Culture Serum
• 293T Cell Culture Serum
• HEK293E (293 c18) Cell Culture Serum
• HEK293S GnTI Cell Culture Serum
• HEK293-FT Cell Culture Serum
• 5637 Cell Culture Serum
• 786-O Cell Culture Serum
• A-375 Cell Culture Serum
• A-431 Cell Culture Serum
• A-498 Cell Culture Serum
• A549 Cell Culture Serum
• ARPE-19 Cell Culture Serum
• BEAS-2B Cell Culture Serum
• BEL-7402 Cell Culture Serum
• BEL-7404 Cell Culture Serum
• BGC-823 Cell Culture Serum
• BT-474 Cell Culture Serum
• CAL-12T Cell Culture Serum
• Calu-6 Cell Culture Serum
• DLD-1 Cell Culture Serum
• GTL-16 Cell Culture Serum
• NCI-H1299 Cell Culture Serum
• Hacat Cell Culture Serum
• HCC38 Cell Culture Serum
• HCC1937 Cell Culture Serum
• HCC827 Cell Culture Serum
• HCT 116 Cell Culture Serum
• HeLa Cell Culture Serum
• Hep 3B Cell Culture Serum
• Hep G2 Cell Culture Serum
• HGC-27 Cell Culture Serum
• HL-60 Cell Culture Serum
• HLE Cell Culture Serum
• HT1080 Cell Culture Serum
• HT-29 Cell Culture Serum
• Hutu80 Cell Culture Serum
• JIMT-1 Cell Culture Serum
• Jurkat Cell Culture Serum
• Kasumi-1 Cell Culture Serum
• KATO III Cell Culture Serum
• KYSE520 Cell Culture Serum
• LNCaP Cell Culture Serum
• LNCaP clone FGC Cell Culture Serum
• MCF7 Cell Culture Serum
• MDA-MB-231 Cell Culture Serum
• MeWo Cell Culture Serum
• MFE280 Cell Culture Serum
• MFE296 Cell Culture Serum
• MGC-803 Cell Culture Serum

Storage

Store at -15 to -40°C, valid for 5 years; typically not recommended to exceed 1 month when stored at 4°C.

Precaution

• If unable to use within a short period, please appropriately aliquot after thawing. When serum freezes, its volume increases by approximately 10%. Therefore, when aliquoting serum, leave some volume space in the aliquot bottles to prevent them from cracking due to freezing, which can lead to contamination.
• Heat inactivation refers to heating thawed serum at 56°C for 30 minutes. Shake regularly during heating. The purpose of heat treatment is to inactivate complement in the serum. Unless necessary, heat treatment of serum is generally not recommended because it significantly increases the precipitation of serum proteins and may affect serum quality. Reactions involving complement include cytotoxicity, smooth muscle contraction, histamine release from mast cells and platelets, enhanced phagocytosis, and promotion of chemotaxis and activation of lymphocytes and macrophages.
• Thawing of bottled serum should be done using a slow thawing method: Place the serum stored in a freezer at -15 to -40°C in a 4°C refrigerator for approximately 1 day to thaw completely before aliquoting. Shake gently every 2 hours during the thawing process (try to avoid bubble formation) to ensure uniform temperature and composition and reduce precipitation. Do not thaw serum directly from -20°C or lower temperatures in a 37°C water bath, as this rapid temperature change may cause protein aggregation and precipitation, resulting in decreased serum quality.
• The flocculent precipitate in the serum is mainly caused by the denaturation of fibrinogen in the serum after thawing and the denaturation of lipoproteins in the serum after long-term storage at 4°C. These flocculents do not affect the quality of the serum itself and may not need to be removed. If necessary, centrifuge at 400g for 5 minutes to remove. However, it is not advisable to filter them out as they may clog the filter membrane.
• The precipitate in heat-treated serum will significantly increase. Some precipitates may appear as "small black dots" under a microscope, and due to the Brownian motion of these "small black dots" magnified under a microscope, they may appear to be moving, leading to mistaken identification as microbial contamination. Typically, these small black dots do not affect cell growth. However, if microbial contamination is suspected, usage should be stopped immediately, and another batch of serum should be used. A suitable amount of serum should be diluted to a 10% concentration in culture medium and cultured for 1-3 days to observe whether there is a sharp increase in small black dots or streak the serum on an LB agar plate for colony observation to confirm microbial contamination.
• Do not leave serum at 37°C for an extended period; otherwise, the serum will gradually become turbid, and the active components in the serum will gradually deactivate, affecting serum quality.
• This product is intended for scientific research by professionals only and must not be used for clinical diagnosis or treatment, food or drug applications, or stored in residential households.
• For your safety and health, please wear lab coats and disposable gloves during handling.

SBS Genetech is recognized as one of the global major leading industry players in Transfection Reagent by third-party market researchers. For more details, please visit Global Transfection Reagent and Equipment Market – Industry Trends and Forecast to 2030.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory