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Cat. No.: Cas9NG-500 (for 500pmol)

Cat. No.: Cas9NG-1k (for 1000pmol)

Description 

SpCas9 nuclease originates from S. pyogenes. It is an RNA-guided endonuclease that can catalyze the specific cleavage of double-stranded DNA. It recognizes the NGG sequence of the PAM (Protospacer Adjacent Motif) and cleaves at the 3rd base of the target sequence. The NGG of the PAM sequence must be located after the gene editing target site, on the complementary strand of the DNA strand complementary to the sgRNA. 

In order to increase the PAM sequence promiscuity, the SpCas9 nuclease was modified to obtain a mutant protein called SpCas9-NG. It can recognize PAM sequences composed of NG, which greatly expands the range of selectable gene editing sites. 

The SpCas9-NG nuclease produced by our company is a recombinant protein expressed in E. coli and purified through multiple steps. The purity is over 90%. The N-terminus of the SpCas9-NG protein has a His tag and TEV protease cleavage site, and no nuclear localization signal. Therefore, it cannot be used for gene editing by transfecting or microinjecting cells. Our company also has SpCas9-NG with nuclear localization signal (NLS).

Advantages

Compared with the mRNA or plasmid systems:

Applications

• In vitro DNA cleavage

• In vitro DNA detection

Storage