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Cat. No.: SRPE-1 (for 1mg)

Cat. No.: SRPE-5 (for 5mg)

Description

SMCC Activated R-Phycoerythrin (SMCC-RPE), also known as SMCC Activated RPE, SMCC Activated R-PE, SMCC-PE, or SMCC Activated Phycoerythrin, is formed by modifying R-Phycoerythrin (R-PE) with Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC). This modification allows R-PE to conjugate with molecules containing thiol groups, such as proteins. The conjugates are commonly used in fluorescent microscopy detection, fluorescent immunoassays, dual or multi-color fluorescence analysis, cancer cell surface antigen detection, flow cytometry, antibody fluorescence labeling, in vivo imaging, and various diagnostic and bioengineering techniques.

RPE is a highly fluorescent phycobiliprotein extracted from red algae, with a molecular weight of approximately 240 kDa. It exhibits an extremely high quantum yield, emitting bright reddish-orange fluorescence that is stable, long-lasting, and highly water-soluble. Compared to conventional organic fluorophores, RPE is brighter and more stable, making it a popular fluorescent label in biological detection. RPE's maximum excitation wavelength is 565 nm, with secondary excitation wavelengths at 480 nm and 545 nm, and a maximum emission wavelength at 575 nm.

SMCC is a common amine-thiol crosslinker containing an amine-reactive N-hydroxysuccinimide (NHS) ester group and a thiol-reactive maleimide group. Under pH 7-9 conditions, the NHS ester group reacts with the primary amines of RPE to form stable amide bonds, while the maleimide group can react with thiols on other molecules, such as proteins, under pH 6.5-7.5 conditions to form stable thioether bonds. The cyclohexane ring in SMCC significantly reduces the hydrolysis rate of the maleimide group, allowing SMCC-modified proteins to be stored in a lyophilized form.

This product is a conjugate of RPE and SMCC, purified and free of excess SMCC, dissolved in PBS, and finally provided in a lyophilized powder form. It retains thiol reactivity even with long-term storage, ensuring optimal conjugation of SMCC-RPE with other thiol-containing molecules such as proteins.

This product can be conjugated to biological macromolecules such as antibodies, which contain cysteine residues with thiol groups. When the thiol groups on cysteine form disulfide bonds, they can be treated with reducing agents to generate free thiol groups. When biological macromolecules such as proteins have available primary amine groups, the required thiol groups can also be added through chemical reactions.

Storage

Store at 4ºC protected from light, valid for one year. Do not store at -20ºC or lower temperatures. After preparing the stock solution with PBS, store at 4ºC protected from light and use within two weeks.

Precautions

• Fluorescent dyes are subject to quenching, so store and use them protected from light to slow down the quenching process.
• After preparing the solution, store it at 4ºC protected from light and do not freeze. The conjugate products should also be stored at 4ºC protected from light and not frozen.
• Avoid using buffers containing primary amines and thiols during the reaction to minimize unnecessary competitive reactions.
• This product is intended for scientific research use by trained professionals only. It is not for clinical diagnosis or treatment, food, or drug use, and should not be stored in residential areas.
• For your safety and health, please wear a lab coat and disposable gloves while handling.

Related: SMCC Activated Allophycocyanin (SMCC-APC)

Only for research and not intended for treatment of humans or animals

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