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Cat. No.: SPMR-50 (for 50T)

Cat. No.: SPMR-200 (for 200T)

Description

In recent years, extensive research on RNA interference and regulatory small RNAs has highlighted an urgent need for a kit that can efficiently extract RNA of approximately 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods, such as silica gel membranes, cannot effectively adsorb and recover these molecules. Phenol/guanidine extraction and isopropanol or ethanol precipitation also cannot effectively precipitate and recover small molecule RNAs. Extracting from serum and plasma samples is even more challenging due to their inherent characteristics.

This kit uses a unique lysis solution to quickly and directly lyse serum and plasma RNases. It employs a robust organic extraction to remove proteins and DNA. RNA, including small molecule RNA, is adsorbed onto a special silica-based membrane in the centrifuge column under high ethanol concentration. This is followed by a series of rapid wash-centrifugation steps using a special washing solution. The wash solution further removes cellular metabolites, proteins, and other impurities. Finally, the RNA is eluted from the silica-based membrane using a low-salt elution solution.

Features

• The silica-based membranes inside the centrifuge adsorption columns are all custom-made by a world-renowned company. The adsorption capacity varies very little between columns, ensuring good repeatability. This overcomes the drawback of inconsistent membrane quality found in ordinary kits.
• There is no need for steps like ethanol precipitation, which can easily lead to the loss of small molecule RNA.
• The kit contains a special lysis solution formula, allowing the processing of more serum/plasma samples.
• Multiple column washes ensure high purity, making the product suitable for RNAi, RT-PCR, Northern-blot, and various other experiments.

Storage

This kit can be stored at room temperature for 6 months without affecting its performance.