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Cat. No.: SPDCP-2 (for 2ml)

Cat. No.: SPDCP-15 (for 15ml)

Description

SDS-PAGE Dual Color Protein Sample Loading Buffer (5X, Odorless) is an improved, safer, and odorless protein loading buffer that uses blue and red dyes. It is a 5-fold concentrated solution.

Features

• This product is safer and healthier. It uses odorless, more water-soluble, and similarly reducing agents to replace the smelly dithiothreitol (DTT) or 2-mercaptoethanol, ensuring that this SDS-PAGE protein loading buffer does not emit any odor during normal use or heating, making the protein loading process safer and healthier.
• Apart from being odorless and having different dyes, this product performs the same as conventional protein loading buffers. It can be used for loading conventional SDS-PAGE protein samples. No significant differences have been observed in its performance compared to regular SDS-PAGE protein loading buffers.
• For conventional SDS-PAGE electrophoresis, the migration rate of the blue dye in this product is consistent with bromophenol blue, and the red dye migrates at a similar speed to the blue dye. In the Tris-Gly electrophoresis system, when the gel concentration is below 15%, the red dye migrates slightly faster than the blue dye, and when the gel concentration is above 15%, the red dye migrates slightly slower than the blue dye.
• This product can be used for lane tracking after membrane transfer: This product contains a special red dye that can be transferred with the protein to PVDF or NC membranes, indicating lane positions and facilitating membrane cutting and other operations.

Precautions

• When using this product for protein denaturation, it is recommended to heat it in a 95°C water bath or PCR instrument for 5 minutes. Excessive temperature (such as 100°C) or prolonged time (such as more than 15 minutes) may cause protein degradation or abnormal color of the indicator in the loading buffer.
• This product does not contain highly toxic mercaptoethanol or DTT with an irritating odor, but the reduction effect is consistent, and the treatment effect on protein samples and electrophoresis is the same.
• This product must be completely dissolved before use.
• This product is intended for scientific research by professionals only and must not be used for clinical diagnosis or treatment, food or drugs, and must not be stored in ordinary residences.
• For your safety and health, please wear a lab coat and disposable gloves when handling.

Storage

Store at -20°C, valid for at least one year.

Related: SDS-PAGE Protein Sample Loading Buffer (5X, Odorless)

Only for research and not intended for treatment of humans or animals

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