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Cat. No.: TSG101-50 (for 50μl)

Source: Rabbit

Application: WB (Western Blot)

Cross-reactivity: H (Human), M (Mouse), R (Rat)

Molecular Weight: ~44 kDa

Description

SBSExo™ Antibody Series are high-specificity, reliable antibodies validated by Western blotting for detecting exosome marker proteins. These antibodies effectively support the study of exosome dynamics and morphology and help elucidate the roles of exosome marker proteins in many exosome-mediated or exosome-influenced functions. Hsp70, CD36, and TSG101 are common exosome protein markers, also known as exosomal markers.

Exosomes are membrane-bound extracellular vesicles (EVs) with diameters of approximately 40-160 nm and a lipid bilayer structure. They naturally occur in blood, urine, cerebrospinal fluid, and the supernatant of cultured cells. Almost all cell types can produce and release exosomes. As shown in Figure 1, the cell membrane undergoes endocytosis, sequentially forming early sorting endosomes (ESE), late sorting endosomes (LSE), and multivesicular bodies (MVB), which contain intraluminal vesicles (ILVs). MVBs fuse with the cell membrane to form exosomes. These exosomes carry various components from their parent cells, including nucleic acids, proteins, lipids, carbohydrates, and metabolites, and release them into the extracellular matrix. Exosomes can be recognized and fused by nearby or distant cells, acting as important mediators of intercellular regulation. They participate in the pathogenesis of various diseases, including cancer, neurodegenerative diseases, and inflammatory diseases, affecting many cellular functions.

Specification

50μl

About this Antibody

Name: TSG101 Rabbit Monoclonal Antibody

Category: Rabbit Monoclonal Antibody (RabMAb); Primary antibody

Isotype: IgG

Purification: Affinity purification

About the Immunogen

Immunogen: A synthetic peptide of human TSG101

Gene ID: 7251

SwissProt: Q99816

Synonyms: TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101

Category: Cell Biology

Background:

Component of the ESCRT-I complex, a regulator of the vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55.

Storage

Store at -20ºC, valid for one year.

Precautions

• It is recommended to use Exosome Isolation Kit from Cell Culture Media (Precipitation Method) for exosome extraction.
• Please recycle used diluted antibodies. Recycled antibodies can typically be reused at least 1-5 times. Store diluted or recycled antibodies at 4ºC.
• Recycled antibodies should be used in the same manner as freshly diluted antibodies. If slight turbidity is observed during reuse, centrifuge at 10,000×g for 1-3 minutes and use the supernatant for subsequent tests. If there are noticeable flocculent particles or microbial growth, consider discarding the antibody.
• This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, food or drugs, or stored in a regular household.
• For your safety and health, wear a lab coat and disposable gloves while handling.

Related:

• SBSExo™ CD9 Rabbit mAb (Exosome Validated)
• SBSExo™ TSG101 Rabbit mAb (Exosome Validated)
• SBSExo™ CD63 Rabbit mAb (Exosome Validated)
• SBSExo™ Hsp70 Rabbit mAb (Exosome Validated)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory