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Cat. No.: SC2MP-300 (for 300 μg)

Cat. No.: SC2MP-1k (for 1 mg)

Description

The SARS-CoV-2 Main Protease differs from the SARS-CoV-2 by only 12 amino acids, sharing over 96% homology, with their structures being virtually identical. The Main Protease plays a critical role in mediating viral replication and transcription, making it an important drug target. This product is purified from an E. coli strain overexpressing the SARS-CoV-2 Main Protease gene (Gene ID: 43740578). During expression, the N-terminal RGSSAVLQSG sequence is recognized and cleaved by the Main Protease. The C-terminal His tag is removed during purification using rhinovirus 3C protease, ensuring that no extra amino acids are present at either end of the peptide chain, matching the amino acid sequence of the SARS-CoV-2 Main Protease.

Product Use

Screening of novel coronavirus inhibitors, enzyme activity studies, or structural research.

Usage Recommendations

1. Optimal temperature: 37°C
2. Optimal pH: 7.3

Storage 

Shelf life: 1 year. Storage at -20°C. For long-term storage, avoid repeated freeze-thaw cycles and store at -80°C.

Quality Assurance

Purified through multiple columns, SDS-PAGE analysis shows a clear single target band with 90% purity.

Activity Definition

One unit of enzyme activity is defined as the amount of enzyme that catalyzes the formation of 1 μmol of MCA-AVLQ per minute at pH 7.0 and 37ºC.

Storage Buffer

• 20 mM Tris-HCl
• 150 mM NaCl
• 1 mM EDTA
• 1 mM DTT
• 50% Glycerol, pH 7.8

Heat Inactivation

75°C for 10 minutes.

Notes

1. Centrifuge appropriately before use to collect protein at the bottom of the tube.
2. This product is for scientific research by professionals only and is not intended for clinical diagnosis or food use.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory