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Cat. No.: RNASER-500 (for 500U)

Cat. No.: RNASER-5k (for 5KU)

Description

Ribonuclease R (RNase R) comes from E.coli, obtained by recombinant expression of gene engineering. This enzyme is an Mg²⁺ dependent 3' - 5' exoribonuclease, which can digest almost all linear RNAs substrates but cannot digest circular RNA, lariat RNA, and double-stranded RNA with 3' protruding ends <7nt. RNase R nuclease exonuclease can efficiently remove linear RNA without secondary structure, so as to purify circular RNA molecules, which is convenient for subsequent RNA sequencing and other experimental studies.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μg of poly-r (A) product at 37°C under the conditions of 20 mM Tris HCl (pH7.5), 100 mM KCl and 0.5 mM Mg²⁺.

Components

RNase R (20 U/μl): 25 μl/250 μl

10×RNase R Buffer: 1 ml

Applications

• Enrichment of cyclic RNA in biological samples
• Analysis and identification of lariat intronic sequences
• Cyclic RNA studies
• Research on alternative splicing

Storage

The minimum shelf life is 3 years at -20°C. Avoid repeated freeze-thaw cycles.

Instruction: Protocol