RDA (RNase H Depended Amplification) Lyophilized Microbeads
$151.20 - $756.00
$840.00
Cat. No.: RDABM-48 (for 48T)
Cat. No.: RDABM-480 (for 480T)
All products have special prices for bulk purchase, please contact for more details if required.
Description
RDA Lyophilized Microbead is a ready-to-use freeze-dried pellet, containing Bst DNA Polymerase, reverse transcriptase, RNase H2, Mg2+, dNTPs, buffering salts, freeze-drying protectants (Poly A and T.E.T), etc. When in use, simply add primers, templates, or dyes, making the operation steps simple.
Utilizing Bst DNA Polymerase modified with an adapter can effectively reduce nonspecific amplification.
Note:
- No visible dyes are added. Experimenters may customize dye combinations or detection methods for the reaction as needed;
- Probes need to be RNA modified. PCR primers, LAMP primers, and RPA primers can seamlessly transition to the RDA amplification system;
- After LAMP primers are RNA modified, amplification efficiency is greatly increased, and detection can be completed within 10 minutes; sensitivity far exceeds PCR;
- This reagent contains dUTP-UDG, effectively avoiding aerosol contamination.
Features
This product is a Mix freeze-dried pellet, containing Bst DNA Polymerase, reverse transcriptase (RTase), RNase H2, Mg2+, dNTPs, buffering salts, freeze-drying protectants (Poly A and T.E.T), etc., required for isothermal amplification.
When in use, only primers, templates, dyes, or probes (RNA modified) need to be added to perform nucleic acid isothermal amplification; this system has been optimized with a special formulation to be compatible with RT-LAM/LAMP systems.
No visible dyes are added to this product. Experimenters may customize dye combinations or detection methods for RDA or LAMP reactions as needed, including using OG dye tubes for orange-green color change, using labeled oligos for strip tests, using fluorescent dyes for fluorescence detection, or using Molecular Beacon probes for fluorescence detection, etc.
Introduction of the heat-sensitive UNG-dUTP system avoids DNA aerosol contamination.
Storage
This product should be transported and stored at 2~28°C, in dry conditions, and protected from light. The shelf life is one year.
Related:
- qPCR Lyophilized Microbeads
- qRT-PCR Lyophilized Microbeads
- LAMP Basic Lyophilized Microbeads
- RT-LAMP Basic Lyophilized Microbeads
- WarmUp LAMP Lyophilized Microbeads
- WarmUp RT-LAMP Lyophilized Microbeads
- Rapid qPCR Lyophilized Microbeads
- Ultra-sensitive qRT-PCR Lyophilized Microbeads
- RDA (RNase H Depended Amplification) Lyophilized Microbeads
- WarmUp RDA (RNase H Depended Amplification) Lyophilized Microbeads
Further Reading
Experience Enhanced Sensitivity and Specificity in RT-LAMP Reactions: Bst DNA/RNA Polymerase
At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst Polymerase and extremely thermostable reverse transcriptase.
Bst DNA/RNA Polymerase is suitable for isothermal amplification reaction of both DNA and RNA templates, which can detect low-sensitivity nucleic acid templates with great efficiency and specificity. Besides, with a special preparation process, this enzyme has a fast amplification rate and high tolerance to impurity. Since our Bst DNA/RNA Polymerase is extremely thermostable and also provides sensitive reverse transcriptase activity, it is reported to have higher sensitivity at high Ct values.[1]
[1] Lu S, Duplat D, Benitez-Bolivar P, León C, Villota SD, Veloz-Villavicencio E, et al. (2022) Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application. PLoS ONE 17(5): e0268340. https://doi.org/10.1371/journal.pone.0268340
Below are the lyophilized LAMP microbeads using Bst DNA/RNA Polymerase:
- PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads
- PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads
- PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads
The World Leading Standard for LAMP Reaction: Bst P DNA/RNA Polymerase
Bst P DNA/RNA Polymerase is an upgraded version of Bst DNA/RNA Polymerase through enzyme electronic re-structure and evolution screening (in silico Design & in vitro Evolution), which is generally used for LAMP or RT-LAMP amplification of DNA or RNA.
Performance improvements include:
- The whole Bst P DNA/RNA System includes hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic facilitates the establishment of the reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures.
- The reaction temperature is further raised to 70°C, which greatly reduces the formation of primer dimer, improves the amplification specificity, and makes the nucleic acid release of crude samples more sufficient.
- The whole portfolio contains Helicase, so Premium LAMP amplification (pLAMP) is allowed without using F3/B3 primers. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This will further reduce non-specific amplification and greatly improve amplification homogeneity.
Below are the lyophilized LAMP microbeads using Bst P DNA/RNA Polymerase:
- PrimeIAmp™ HotStart Bst P Basic Microbeads
- PrimeIAmp™ HotStart Bst P SYBR Green Microbeads
- PrimeIAmp™ HotStart Bst P L-HNB Microbeads
SBS Genetech is recognized as one of the global major leading industry players in Lyophilized Reagent Beads by third-party market researchers. For more details, please visit Global Lyophilized Reagent Beads Market Research Report 2024.
