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Cat. No.: NBBD-2k (for 2KU)

Recognition Site

GCTCTTCN
5'...G C T C T T C N↓...3'
3'...C G A G A A G N ...5'

Description

We are proud to introduce RapidCleave™ Fast Restriction Enzymes, a meticulously engineered series of rapid restriction endonucleases. Whether you're working with plasmid DNA, PCR products, or genomic DNA, RapidCleave™ delivers astonishing enzyme cleavage speed and outstanding performance. Whether you're a seasoned lab professional or a newcomer, RapidCleave™ Fast Restriction Enzymes will make it easier for you to achieve your research goals, accelerate scientific progress, and inject new vitality into your laboratory work. Experience RapidCleave™ for unprecedented DNA cleavage speed and convenience.

Nt.BspQI is a type of nicking endonuclease that cleaves only one strand of the dsDNA substrate; it generates a nick on the dsDNA substrate without cleaving the dsDNA.

Recommended Reaction Conditions

• 1× RapidCleave™ Buffer;
• Incubation at 65°C;
• Refer to the "DNA Rapid Cleavage Protocol" for reaction setup.

Inactivation Conditions

Incubate at 80°C for 20 minutes.

Methylation Sensitivity

• Cutting may be hindered for DNA methylated by EcoBI.