Rapid TUNEL Apoptosis Detection Kit
$800.00
$1,000.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: RTADK-100 (for 100T)
Description
Rapid TUNEL Apoptosis Detection Kit is used to detect DNA fragmentation in cells undergoing apoptosis. The principle of this assay involves biotin-labeled dUTP being incorporated into the 3'-OH ends of fragmented DNA in apoptotic cells by the enzyme terminal deoxynucleotidyl transferase (TdT). The biotin-labeled dUTP can then specifically bind to streptavidin-horseradish peroxidase (Streptavidin-HRP), and in the presence of the HRP substrate diaminobenzidine (DAB), a strong color reaction (deep brown) is produced. This allows for the specific and accurate localization of apoptotic cells, making them observable and countable under a standard microscope. Normal or proliferating cells, which exhibit minimal DNA fragmentation, do not have 3'-OH ends and are rarely stained. This kit is suitable for in situ detection in tissue samples (paraffin-embedded, frozen, and ultra-thin sections) and cell samples (cell smears or coverslips).
Components
100T
- Equilibration Buffer
- TdT Enzyme
- Biotin-11-dUTP
- Streptavidin-HRP
Additional Notes
Transportation and Storage Conditions: Transport at 2-8°C. Upon receipt, store at -20°C. Shelf life is one year.
Sample Labeling: Please label each sample with an immunohistochemistry pen. Additionally, prepare two slides of sample tissue, one for positive control and one for negative control, and label them accordingly.
Preparation of Working Solutions: Prepare the working solutions for the next steps during the intervals between each reaction or wash.
Preparation of Reaction Solutions: It is recommended to prepare the reaction solutions in bulk according to the number of samples to avoid reagent loss from individual preparation for each sample. If the TdT enzyme reaction solution needs to be stored temporarily, keep it on ice.
Safety Precautions: Wear gloves during the procedure to prevent reagent contact with skin. If contact occurs, rinse immediately with plenty of water.
Special Tissue Samples: For special tissues such as brain or heart, it is recommended to use Rapid TUNEL Apoptosis Detection Kit (High-Sensitivity).
Tissue Sample Preparation:
Paraffin Samples: After perfusing the animal with 4% paraformaldehyde, promptly collect the tissue and transfer it to 15 mL or 50 mL sealed centrifuge tubes. Fix in 4% paraformaldehyde (tissue must be completely immersed in the fixative) at room temperature for at least 24 hours. The samples can be stored for long periods and transported at room temperature. Ensure the sealing film is properly sealed. Tissue blocks should not be excessively large or thick; generally, they should be 2 cm x 1.5 cm x 0.3 cm, with the thickness preferably not exceeding 0.3 cm (prepare fixative solution fresh).
Frozen Samples: After perfusing the animal with 4% paraformaldehyde, promptly collect the tissue, fix in 4% paraformaldehyde for 24 hours, dehydrate in 30% sucrose for more than 48 hours, and freeze at -20°C or -80°C. For special tissues (e.g., brain tissue requires perfusion before collection; eyes require special fixatives).
Storage
Store at -20°C for 12 months.
Only for research and not intended for treatment of humans or animals
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