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Cat. No.: PGPMB-1 (for 1ml)

Cat. No.: PGPMB-5 (for 5ml)

Description

Protein G Plus Magnetic Beads, also known as Protein G immunomagnetic beads, Protein G immunoprecipitation magnetic beads, SPG magnetic beads, Protein G magnetic beads, Protein G immunomagnetic beads, or Protein G immunoprecipitation magnetic beads, are covalently coupled with optimized and high-quality recombinant Protein G and nano-sized amino magnetic beads. Compared to Protein G Magnetic Beads, Protein G Plus Magnetic Beads exhibit a stronger ability to specifically bind to corresponding antibodies. Under the same usage of magnetic beads, Protein G Plus Magnetic Beads can bind approximately 30-50% more antibodies. They are mainly used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), chromatin immunoprecipitation (ChIP), as well as antibody purification from samples such as serum, cell culture supernatant, or ascites.

Protein G is an immunoglobulin-binding protein expressed by C-type or G-type streptococcal bacteria. Protein A is a cell wall surface protein found in Staphylococcus aureus, with a molecular weight of 42 kDa. Protein G and Protein A have similar functions and can specifically bind to mammalian immunoglobulins (Ig). The binding sites are usually located in the Fc region of immunoglobulins, although there is evidence that Protein A can also bind to the Fab region of the human VH3 family, and Protein G sometimes has certain binding to the Fab region as well. Additionally, they have different binding abilities for different subclasses of immunoglobulins. Recombinant modified Protein A and Protein G can be combined with magnetic beads in a certain way for immunoprecipitation or antibody purification. The Protein G Plus used in this magnetic bead retains the basic properties of Protein G while optimizing the steric hindrance effect for simultaneous binding to three IgG molecules. It exhibits a stronger ability to specifically bind to corresponding antibodies, enabling it to bind approximately 30-50% more antibodies under the same usage of magnetic beads.

Similar to Protein G Magnetic Beads, Protein G Plus Magnetic Beads are suitable for immunoprecipitation of human IgG1, IgG2, IgG3, IgG4, mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, as well as rabbit and goat polyclonal antibodies, while Protein A Magnetic Beads are suitable for immunoprecipitation of human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, and rabbit IgG, etc.

The recombinant Protein G Plus in this product can specifically bind to the Fc region of most mammalian IgG antibodies, with a molecular weight of approximately 25 kDa. This recombinant Protein G Plus has been modified to retain only the amino acid sequences related to the binding of IgG Fc region, while removing sequences outside the binding sites that may lead to non-specific binding, thereby effectively reducing non-specific binding. Each Protein G Plus molecule in this product can bind to three IgG molecules.

Protein G Plus Magnetic Beads can specifically bind to corresponding antibodies and can be conveniently applied in immunoprecipitation or antibody purification of target proteins or protein complexes using magnetic separation devices such as magnetic racks. The table below summarizes the binding capabilities of our Protein A, Protein G, and Protein A/G Magnetic Beads products with common immunoglobulin subclasses in humans, mice, and rats, as well as their overall binding capabilities across different species.

• ++++, Strong Binding
• ++~+++, Medium Binding
• +, Weak Binding
• +/-, Weak or No Binding
• -, No Binding

The recombinant Protein G Plus in this product can specifically bind to the Fc region of most mammalian IgG, with a molecular weight of approximately 25 kDa. This recombinant Protein G Plus has been engineered to retain only the amino acid sequences related to IgG Fc binding, while removing sequences outside the binding sites that could cause nonspecific binding, effectively reducing nonspecific interactions. Each Protein G Plus molecule in this product can bind to 3 IgG molecules.

Protein G Plus Magnetic Beads can specifically bind to corresponding antibodies and, with the help of magnetic separation devices such as magnetic racks, can be conveniently used for immunoprecipitation (IP) of the target protein or its protein complex bound to the antibody, as well as for antibody purification experiments.

Features

• This product has strong antibody binding specificity and higher antibody binding capacity. Traditional Protein G agarose has a larger pore size, which is prone to nonspecific adsorption, while the magnetic beads in this product have smaller particle size, reducing nonspecific adsorption. Each milliliter of magnetic bead suspension contains approximately 10mg of magnetic beads, with no less than 0.6mg of recombinant Protein G Plus. Each recombinant Protein G Plus molecule has three Fc binding domains, typically allowing for binding of not less than 0.9mg of human IgG per milliliter of the magnetic bead suspension. The specific maximum binding capacity may vary depending on the antibody type and target protein. For a 500μl sample, usually only 10-20μl of the magnetic bead suspension is needed for efficient immunoprecipitation experiments.
• This product offers fast binding of antibodies or antibody complexes. The nanoscale magnetic beads (~200nm) used in this product have a large surface area, facilitating rapid and efficient binding of antibodies or antibody complexes. Typically, the adsorption process of antibodies or their complexes can be completed within 10 minutes, and the immunoprecipitation of the target protein can be completed within 30 minutes. Shortening the operation time helps to avoid degradation or denaturation of the target protein during prolonged procedures, ensuring the activity of the target protein. Magnetic separation is employed, allowing for a 40% time-saving compared to agarose gel when performing IP and Co-IP experiments.
• This product offers multiple options for elution methods. Depending on the structure of the target protein in the antibody complex, its biological function, and the requirements for subsequent applications, various elution methods can be used, including acidic solutions, SDS-PAGE sample buffer, or competitive peptides as elution buffers.
• This product is convenient to use. It is stored in a special protective solution without glycerol and can be rapidly and efficiently separated using magnetic adsorption, without the need for centrifugation.

Precautions

• This product has been tested and can withstand repeated freezing and thawing for more than three cycles without affecting its performance.
• It is important to maintain the pH of the product between 6-8 and avoid high-speed centrifugation, drying, or freezing. Do not leave the magnetic beads in a magnetic field for an extended period as it may cause bead aggregation.
• Before using the product, it should be properly resuspended by gently inverting several times to ensure uniform mixing of the magnetic beads. Avoid vigorous vortexing or shaking, as it may lead to protein denaturation.
• During immunoprecipitation or purification, it is recommended to include positive and negative control groups.
• Protein samples should be purified as soon as possible after collection and kept at 4°C or on ice to slow down protein degradation or denaturation. To effectively inhibit protein degradation, an appropriate amount of protease inhibitor cocktail can be added to the protein sample.
• When using equipment such as a vacuum pump to aspirate the supernatant, be cautious of the suction strength to avoid aspirating aggregated magnetic beads.
• Aggregation of magnetic beads may occur during acid elution, which is a normal phenomenon and does not affect the normal use of the beads. The addition of 0.1% non-ionic detergent (such as Triton X-100, Tween-20, or NP-40) can effectively prevent bead aggregation without affecting the antibody binding efficiency of the magnetic beads.
• High concentrations of DTT, 2-mercaptoethanol, guanidine hydrochloride, and similar substances may have an impact on the binding of this product to labeled proteins.
• This product is intended for scientific research purposes by professionals only and should not be used for clinical diagnosis or treatment. It should not be used for food or drugs and should not be stored in a regular household.
• For your safety and health, please wear appropriate laboratory attire and disposable gloves when handling the product.

Storage

Store at 4°C, valid for two years. If not used for an extended period, it can be stored at -20°C, which allows for longer storage time.

Related:

• Protein A Magnetic Beads
• Protein A/G Magnetic Beads
• Protein G Magnetic Beads
• Protein G Plus Magnetic Beads
• Protein L Magnetic Beads

Only for research and not intended for treatment of humans or animals

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