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Cat. No.: PAGFA-10 (for 10ml)

Cat. No.: PAGFA-50 (for 50ml)

Cat. No.: PAGFA-200 (for 200ml)

Description

Protein A+G Agarose (Fast Flow, for antibody purification) is a fast-flow agarose resin that combines Protein A and Protein G. It is also known as Protein A+G Resin (Protein A+G affinity chromatography resin). It is primarily used for antibody purification and can also be used for immunoprecipitation (IP) or co-immunoprecipitation (Co-IP).

For immunoprecipitation or co-immunoprecipitation, Protein A+G Agarose (Fast Flow, for IP) can also be used.

Protein A is a cell wall surface protein found in Staphylococcus aureus with a molecular weight of 42 kDa. Protein G is an immunoglobulin-binding protein expressed by C-type or G-type Streptococcal bacteria. Protein A and Protein G have similar functions and can specifically bind to mammalian immunoglobulins (Ig), usually in the Fc region of the immunoglobulin. However, there is evidence that Protein A can also bind to the Fab region of the human VH3 family, and Protein G can sometimes bind to the Fab region as well. Additionally, their binding capacities vary for different subclasses of immunoglobulins. Recombinant modified Protein A and Protein G are combined with agarose in a specific manner, making them suitable for antibody purification or immunoprecipitation.

Protein A+G Agarose is suitable for purifying all antibodies that can be immunoprecipitated individually with Protein A Agarose and Protein G Agarose, including human IgG1, IgG2, IgG3, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3; rat IgG1, IgG2a, IgG2b, IgG2c; rabbit IgG; and rabbit and goat polyclonal antibodies.

The recombinant Protein A and Protein G in this product can specifically bind to the Fc region of most mammalian IgG antibodies, with molecular weights of 14 kDa and 22 kDa, respectively. Through modification, these recombinant Protein A and Protein G retain only the amino acid sequences relevant to binding with the IgG Fc region, while removing sequences outside the binding sites that may cause non-specific binding. This effectively reduces non-specific binding. Each Protein A molecule and Protein G molecule can bind to 2 and 3 IgG molecules, respectively.

The Protein A and Protein G in this product are covalently coupled to 4% cross-linked agarose resin with high cross-linking and fast flow characteristics. They are mixed in a 1:1 ratio. Each milliliter of Protein A+G agarose beads (resin) is coupled with approximately 10 mg of recombinant Protein A and 1 mg of Protein G. Each milliliter of Protein A+G agarose beads (resin) can bind to over 25 mg of human IgG. The average diameter of the agarose beads in this product is 90 μm. The recommended linear flow rate for antibody purification is 50-300 cm/h, with a maximum pressure tolerance of 0.3 MPa.

This product is formulated in a suitable solution that can be directly used for immunoprecipitation and antibody purification. Each milliliter contains 0.25 ml of agarose beads (resin). The volume indicated for this product refers to the total volume of the suspension.

Precautions

• Do not freeze this product for storage.
• Before using Protein A+G Agarose, it must be thoroughly resuspended by gently inverting several times to ensure a uniform mixture.
• This product contains trace amounts of preservatives that do not interfere with routine antibody purification and immunoprecipitation. However, if subsequent enzymatic activity assays are involved, it is recommended to wash the Protein A+G Agarose beads three times with an appropriate solution such as TBS before use to eliminate any potential interference from the preservatives.
• Starting from the collection of protein samples, all steps involving protein samples must be performed at 4°C or on ice.
• This product is intended for scientific research use by professionals only and should not be used for clinical diagnosis or treatment, food or drug purposes, or stored in ordinary households.
• For your safety and health, please wear lab attire and disposable gloves when handling this product.

Storage

Store at 4°C. Valid for one year. Do not freeze.

Only for research and not intended for treatment of humans or animals

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