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Cat. No.: PICBC-1 (for 1ml)

Cat. No.: PICBC-5 (for 5ml)

Description

Protease Inhibitor Cocktail (for Bacterial Cell Extracts, 100X) is a protease inhibitor mixture used for bacterial protein extraction (23mM AEBSF, 2mM Bestatin, 0.3mM E64, and 2mM Pepstatin A in DMSO), and it includes individually packaged 0.1M EDTA.

Bacterial extracts contain many endogenous proteases and phosphatases, which can easily lead to protein degradation or dephosphorylation in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate protease and phosphatase inhibitors to the extracts is an effective method to prevent protein degradation and dephosphorylation.

This product is an optimized and tested protease inhibitor mixture for bacterial protein extraction, containing broad-spectrum serine, cysteine, and acidic protease inhibitors, as well as aminopeptidase inhibitors.

This product is convenient to use. Typically, a 1:100 ratio of the Protease Inhibitor Cocktail (for Bacterial Cell Extracts, 100X) and 0.1M EDTA is added to the lysis buffer for protein extraction from bacteria, effectively inhibiting protein degradation.

Different types of protein extracts contain different types of proteases. For example, mammalian cell extracts usually contain high levels of serine proteases, bacterial cell extracts often contain more serine and metalloproteases, mammalian tissue extracts mainly contain serine, cysteine, and metalloproteases, and plant extracts commonly contain large amounts of serine and cysteine proteases, with some also containing aspartic proteases. Therefore, we provide different types of protease and phosphatase inhibitor cocktails for different types of sample protein extraction.

This product can effectively inhibit various protease activities in bacterial extracts, such as serine proteases, aminopeptidases, cysteine proteases, threonine and aspartic proteases, and metalloproteases. Among them, AEBSF is a water-soluble, irreversible serine protease inhibitor with an inhibition constant similar to PMSF and DFP, effectively inhibiting proteases such as trypsin, chymotrypsin, plasmin, kallikrein, and thrombin. As a substitute for PMSF and DFP, AEBSF has lower toxicity, better water solubility, and stability in aqueous solutions. Bestatin is a reversible aminopeptidase inhibitor; E64 is an irreversible cysteine protease inhibitor; Pepstatin A is a reversible aspartic protease inhibitor; and EDTA, as a chelating agent, is a reversible inhibitor of metalloproteases.

Storage

Store at -20°C for one year.

Precautions

• This product can be aliquoted and stored appropriately, and should be added to lysis buffer or other suitable solutions just before use.
• This product includes individually packaged EDTA, which can be used selectively. For example, if the extract will be used to detect metalloprotease activity, EDTA should not be added. 
• The protease inhibitors in this product are harmful to humans. Handle with care and use effective protection to avoid direct contact with the body or inhalation. 
• This product is for scientific research use only by professionals. It is not for clinical diagnosis or treatment, not for use in food or drugs, and should not be stored in ordinary residences. 
• For your safety and health, wear a lab coat and disposable gloves when handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory