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Cat. No.: PIC-1 (for 1ml)

Cat. No.: PIC-5 (for 5ml)

Description

Protease Inhibitor Cocktail (100X) is a general-purpose protease inhibitor mixture used for protein extraction from cells or tissues (200mM AEBSF, 30μM Aprotinin, 13mM Bestatin, 1.4mM E64, and 1mM Leupeptin in DMSO), and it comes with separately packaged 0.1M EDTA.

Samples such as cell or tissue extracts contain many endogenous proteases and phosphatases, which can easily lead to protein degradation or de-modification in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate protease and phosphatase inhibitors to extracts and other samples is an effective method to prevent protein degradation and de-modification.

This product is an optimized and tested protease inhibitor mixture for routine protein extraction from cells or tissues, including broad-spectrum serine, cysteine, and acidic protease inhibitors, as well as aminopeptidase inhibitors.

This protease inhibitor mixture contains 200mM AEBSF, 30μM Aprotinin, 13mM Bestatin, 1.4mM E64, and 1mM Leupeptin, formulated in high-purity DMSO. EDTA is packaged separately for selective use. If detecting metalloprotease activity, EDTA should not be added.

This product is convenient to use. Typically, the Protease Inhibitor Cocktail (100X) and 0.1M EDTA are added to the lysis buffer at a ratio of 1:100, respectively, for routine extraction of proteins from cells or tissues, effectively inhibiting protein degradation.

Different types of protein extracts contain different types of proteases. For example, mammalian cell extracts usually contain high levels of serine proteases, bacterial cell extracts often contain more serine and metalloproteases, mammalian tissue extracts mainly contain serine, cysteine, and metalloproteases, and plant extracts often contain large amounts of serine and cysteine proteases, with some containing aspartic proteases. Therefore, we provide different types of protease and phosphatase inhibitor mixtures for different sample types.

This product can effectively inhibit various protease activities in routine cell or tissue extracts, such as serine proteases, aminopeptidases, cysteine proteases, threonine and aspartic proteases, and metalloproteases. AEBSF is a water-soluble, irreversible serine protease inhibitor with an inhibition constant similar to PMSF and DFP, effectively inhibiting proteases such as trypsin, chymotrypsin, plasmin, kallikrein, and thrombin. As a substitute for PMSF and DFP, AEBSF has lower toxicity, better water solubility, and stability in aqueous solutions. Aprotinin is a competitive reversible inhibitor of serine proteases; Bestatin is a reversible inhibitor of aminopeptidases; E64 is an irreversible inhibitor of cysteine proteases; Leupeptin is a reversible inhibitor of serine and cysteine proteases; EDTA, as a chelating agent, is a reversible inhibitor of metalloproteases.

Storage

Store at -20°C, valid for one year.

Precautions

• This product can be aliquoted and stored, and should be added to the lysis buffer or other appropriate solutions just before use.
• This product includes separately packaged EDTA for selective use. For example, if the extract will be used to detect metalloprotease activity, EDTA should not be added.
• The protease inhibitors in this product are harmful to humans. Handle with care and use appropriate protective measures to avoid direct contact with the body or inhalation.
• This product is for scientific research use by professionals only. It is not for clinical diagnosis or treatment, not for use in food or drugs, and should not be stored in ordinary residences.
• For your safety and health, please wear a lab coat and disposable gloves when handling this product.

Only for research and not intended for treatment of humans or animals

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