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Cat. No.: PTTAB-20 (for 20T) 

Cat. No.: PTTAB-80 (for 80T) 

Description

PrimeTopo™ TA-Blunt Cloning Kit is an innovative product that is very different from the traditional T4 Ligase. This kit can connect DNA fragments instantly (seconds to minutes) and efficiently (nearly 100%). This kit is recognized as a new generation of world-leading simple, fast, zero background and, screening-free Topo TA-Blunt cloning kit.

Features

• High Compatibility: Any PCR product (compatible with A-end and blunt-end) can be connected instantly (seconds to minutes).
• High Efficiency: The size of our novel PrimeTopo™ TA-Blunt Vector is less than 2kb, and the plasmid size after ligation is generally 2kb smaller than the traditional vectors. The smaller the plasmid, the higher the transformation efficiency, which greatly improves the number of assembled products after ligation of various fragments.
• Shorter Recovery Time: The recovery time of the Amp resistant vector is only 10 min, which is 6 times shorter than that of the Kana resistant vector.
• Convenient Transformation: The transformation can be completed within 5 min at room temperature without an ice bath and heat shock. The subsequent recovery only takes 15 min.
• Minimal False Positive: Based on the zero-background technology by suicide gene, there is no self-connected false positive, thus no cumbersome Blue-White Screening or Colony PCR Screening is required.
• Ideal for Long Fragments: The ability to connect long fragments of PrimeTopo™ TA-Blunt Vector is far superior to the traditional TA-Blunt cloning vector. It can connect up to 10kb fragments.

Storage

The minimum shelf life is 12 months at -20°C.

Instruction: Protocol

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory