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Cat. No.: PRAMRQ-1 (for 25 RT and 500 qPCR reactions) 

Description

PrimeRT All-in-One miRNA RT & qPCR Detection Kit (25 RT and 500 qPCR reactions) contains all components required for miRNA detection, both RT and qPCR reagents. Using miRNA as a template, cDNA can be synthesized efficiently by adding a poly(A) tail and reverse transcribing in one step. The subsequent detection is achieved by the qPCR master mix. This kit is ideal for various miRNA samples, including total RNA, small RNA, etc.

Features

• The optimum poly(A) polymerase and reverse transcriptase ratio ensure the high efficiency of miRNA detection.
• The addition of poly(A) tail and reverse transcription of cDNA are all completed in one step and a single tube
• qPCR master mix has high efficiency, specificity, and sensitivity.
• The ROX reference dye in the kit satisfies different instruments.

Storage

The minimum shelf life is a year at -20°C.

Instruction: Protocol

Related: PrimeRT First Strand cDNA Synthesis Premix (with gDNA Clean)

Only for research and not intended for treatment of humans or animals

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