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Cat. No.: ILHPMB-100 (for 100T)

Cat. No.: ILHPMB-1k (for 1,000T)

Description

PrimeIAmp™ HotStart Bst P L-HNB Microbeads is a full-system lyophilized microbead product, which contains Bst P DNA/RNA Polymerase, L-HNB (Leuco-HNB) colorimetric dye, Helicase, dNTP, Mg²⁺, reaction buffer salt, lyophilized excipient. The amplification system is 25μl for each microbead.

L-HNB dye in the product combines with Mg²⁺ to produce deep violet color before the reaction starts. With the LAMP reaction, the resulting PPi (inorganic pyrophosphate) combines with Mg²⁺ in the system, making L-HNB show light green.

Features

• The whole Bst P DNA/RNA System includes hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic facilitates the establishment of the reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures.
• The reaction temperature is further raised to 70°C, which greatly reduces the formation of primer dimer, improves the amplification specificity, and makes the nucleic acid release of crude samples more sufficient.
• The whole portfolio contains Helicase, so Premium LAMP amplification (pLAMP) is allowed without using F3/B3 primers. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This will further reduce non-specific amplification and greatly improve amplification homogeneity.

Storage

The minimum shelf life is 24 months at -20°C when sealed. The minimum shelf life is 12 months at room temperature (25°C) when sealed. Avoid moisture.

Instruction: Protocol

Related: 

Lyophilized beads based on Bst DNA Polymerase

• LAMP Basic Lyophilized Microbeads
• RT-LAMP Basic Lyophilized Microbeads
• RDA (RNase H Depended Amplification) Lyophilized Microbeads

Lyophilized beads based on WarmUp Bst DNA Polymerase (upgraded version of Bst DNA Polymerase)

• WarmUp LAMP Lyophilized Microbeads
• WarmUp RT-LAMP Lyophilized Microbeads
• WarmUp RDA (RNase H Depended Amplification) Lyophilized Microbeads

Lyophilized beads based on Bst DNA/RNA Polymerase

• PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads
• PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads, 
• PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads

Lyophilized beads based on Bst P DNA/RNA Polymerase (upgraded version of Bst DNA/RNA Polymerase)

• PrimeIAmp™ HotStart Bst P Basic Microbeads
• PrimeIAmp™ HotStart Bst P SYBR Green Microbeads
• PrimeIAmp™ HotStart Bst P L-HNB Microbeads

Customized Lyophilized Microbead Service

Looking for a personalized solution for your research needs? 

Look no further than our customized lyophilized microbead services! We offer lyophilized microbeads with customized primers and reaction volumes of 20-100 μl per bead, ensuring that your experiments are optimized to your exact specifications. Don't settle for a one-size-fits-all approach - Contact us today to learn more about how our customized lyophilized bead services can take your research to the next level!

Viewpoint

Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.

SBS Genetech: Leading the Next Generation Isothermal Amplification Technology

SBS Genetech is recognized as one of the global major leading industry players in Lyophilized Reagent Beads by third-party market researchers. For more details, please visit Global Lyophilized Reagent Beads Market Research Report 2024.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory