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Cat. No.: FPMM-10k (for 10KU) 

Cat. No.: FPMM-100k (for 100KU) 

For M-MLV Reverse Transcriptase active at 65°C, please see Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 55°C, please see Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

Description

Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is modified by the point mutation on the basis of the wild-type M-MLV reverse transcriptase gene and expressed and purified by specially engineered bacteria. On the basis of retaining the activity of the previous enzyme, such as RNA-dependent DNA polymerase activity, DNA-dependent DNA polymerase activity, and non-RNase H activity, it has improved reverse transcription efficiency and heat resistance, making its reverse transcription temperature up to 72°C. Thus, the tolerance to the complex secondary structure of RNA is enhanced. This enzyme has the characteristics of high synthesis capacity, good thermal stability, and a long half-life. Compared with the common mutants obtained by deleting the RNase H domain, this enzyme retains the complete protein structure, so it has the same polymerase activity as the wild type.

Components

• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-) (200 U/µl): 50 μl
• 5 × RT Buffer: 500 μl

Features

• High reverse transcription efficiency
• High heat resistance (active at 72°C)
• High fidelity
• Tolerant to complex secondary structure RNA
• No RNase H activity

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

Related: 

• Mutant M-MLV Reverse Transcriptase
• Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• TGI-RTase Reverse Transcriptase
• Warm Activated Reverse Transcriptase

Only for research and not intended for treatment of humans or animals

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