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Cat. No.: PT7P-10k (for 10KU)

Cat. No.: PT7P-200k (for 200KU)

For the standard version, please visit T7 RNA Polymerase.

Description

Prime T7 RNA polymerase has high specificity for T7 phage promoters and can use it as a promoter and DNA as a template for the synthesis of sense-stranded RNA in vitro. Both linear plasmids and PCR product double-stranded DNA can be used as substrate templates for Prime T7 RNA polymerase. Prime T7 RNA polymerase has been engineered by electronic reconstruction and library screening. Compared to the wild type, the enzyme has a higher affinity for the DNA template binding region, enabling it to have a sustained synthesis ability, resulting in higher yields and easier synthesis of long fragments of RNA. The enzyme is a recombinant purified product without DNase and RNase contamination.

Applications

• Radiolabeled RNA probe preparation
• RNA generation for studies of the RNA structure, processing, and catalysis
• Expression control via anti-sense RNA
• mRNA, sgRNA synthesis

Storage

The minimum shelf life is 2 years at - 20°C. If there are white precipitates, please use the enzyme after a warm bath at 37°C for 5 minutes. This will not affect the performance.

Heat inactivation

75°C，10min

Instruction: Protocol

Related: HY High-Yield T7 RNA Synthesis Kit, RNasin (RNase inhibitor), NTP, DNase I (RNase Free), Thermostable Inorganic Pyrophosphatase

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory