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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: PNGF-15k (for 15KU)

Cat. No.: PNGF-75k (for 75KU)

The glycerol-free version is also available for this product, which is convenient for achieving optimal results in HPLC methods.

Description

PNGase F is an amidase enzyme that cleaves N-linked sugars on glycoproteins. It cleaves various types of sugars, including high-mannose, hybrid, and complex oligosaccharides. The cleavage site is between the innermost N-acetylglucosamine (GlcNAc) and the asparagine residue. PNGase F is also available in a glycerol-free form, which is convenient for achieving optimal results in HPLC methods. 

Activity Definition

One unit of activity is defined as the amount of enzyme required to remove more than 95% of carbohydrates from 10 µg of denatured RNase B in the presence of 1× PNGase reaction buffer at 37°C for 60 minutes. The molecular weight of glycosylated RNase B is approximately 17,000 Da, and it is reduced to approximately 13,683 Da after deglycosylation.

Activity Assay Conditions

• 1× PNGase Buffer: Glycoprotein denaturing buffer (containing 0.1% SDS, 10 mM DTT), 1× GlycoBuffer 2 buffer, 1% NP-40, reaction at 37°C for 1 hour.
• The glycoprotein is denatured by boiling in 1× glycoprotein denaturing buffer (containing 0.5% SDS, 40 mM DTT) at 100°C for 10 minutes.

Applications

• Removal of N-linked sugars on glycoproteins.
• PNGase F can effectively hydrolyze almost all N-linked sugars in glycopeptides/glycoproteins.

Storage

This product can be stored at -20°C for 2 years, avoid repeated freeze-thaw cycles.

Related: 

• PNGase F
• PNGase F (with His-tag)
• PNGase F (with His-tag, Glycerol Free)
• PNGase F Deglycosylation Kit with Magnetic Beads
• Rapid PNGase F Deglycosylation Kit (Denaturing Method)
• Rapid PNGase F Deglycosylation Kit (Native Method)