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Cat. No.: PMSF-5 (for 5g)

Cat. No.: PMSF-25 (for 25g)

Cat. No.: PMSF-100 (for 100g)

General Information

• Name (English):    Phenylmethanesulfonyl fluoride
• Specifications:    Reagent grade, ≥98.5%
• Chemical Formula:    C7H7FO2S
• Synonym:    alpha-Toluenesulfonyl fluoride; Benzylsulfonyl fluoride; PMSF; Phenylmethylsulfonyl fluoride
• Beilstein Registry No.:    2088311
• EINECS Number:    206-350-2
• MDL Number:    MFCD00007424
• UNSPSC Code:    12352202

Application

PMSF is commonly used in lysis buffers during protein separation and preparation processes to inhibit proteases that degrade proteins after cell or tissue lysis, thereby preserving the desired proteins. PMSF is unstable in aqueous solutions. Therefore, it is typically prepared as a stock solution in anhydrous organic solvents such as 100% ethanol or anhydrous isopropanol. The main characteristics and advantages of PMSF include:

• Inhibition of serine proteases such as trypsin and chymotrypsin
• Inhibition of thiol proteases (reversible with reducing thiols) and mammalian acetylcholinesterase
• Lower toxicity compared to DFP (diisopropyl fluorophosphate), but less effective than DFP
• Effective concentration range: 0.1-1 mM
• Half-life at pH 7.5: 1 hour. Phenylmethanesulfonyl fluoride has been used in protein imprinting analysis and radiolabeling.
   

Biochem/physiol Actions

Ingestion of PMSF produces analgesic effects unrelated to its anti-cholinesterase action and prolongs the analgesic effects of centrally administered β-endorphin.

General Description

Phenylmethanesulfonyl fluoride (PMSF) is a widely used serine protease inhibitor that exhibits good inhibitory activity against enzymes such as chymotrypsin, thrombin, and trypsin. PMSF acts as an inhibitor by sulfonating the hydroxyl group of serine residues at the reactive site of serine proteases.

Properties

• biological source:    synthetic
• assay:    ≥98.5%
• form:    powder
• mp:    91-94 °C
• solubility:    dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.) H2O: unstable

Safety Information

• Hazard Pictogram Codes:    GHS05, GHS06
• Signal Word:    Danger
• Hazard Statements:    H301-H314
• Precautionary Statements:    P280-P305 + P351 + P338-P310
• Personal Protective Equipment:    Eyeshields,, Faceshields,, Gloves,, type P1 (EN143) respirator filter,, type P3 (EN 143) respirator cartridges,
• Hazard Codes (Europe):    T
• Risk Codes (Europe):    25-34
• Safety Codes (Europe):    26-36/37/39-45
• RIDADR:    UN 2928 8(6.1) / PGII
• WGK Germany:    3
• RTECS:    XT8040000
• Flash Point (F):    -
• Flash Point (C):    -

Precautions

• This product is for scientific research purposes only and is intended for use by professionals. It is not to be used for clinical diagnosis or treatment, and should not be used in food or drugs. It should not be stored in residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves when handling this product.

Storage

Store at room temperature.

Only for research and not intended for treatment of humans or animals

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