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Cat. No.: PPTK-100 (for 100T)

Cat. No.: PPTK-500 (for 500T)

Description

Plant Protoplasts Transfection Kit can be used to transfect plasmids, Cas9/sgRNA proteins, and RNA into plant protoplasts. After a certain period of cultivation following transfection, the transfected plant protoplasts can be used to detect the expression and function of exogenous genes, or the effects of gene knockdown, knockout, or editing post-transfection.

For Arabidopsis protoplasts, transcriptional changes (such as mRNA and other RNA changes) can typically be detected 2-6 hours after transfection with plasmids using this kit. Changes in protein expression are usually detectable 2-16 hours post-transfection, and gene editing effects can typically be detected within 24 hours.

Plant protoplasts are crucial materials for a range of plant cell studies and can be transfected using methods such as electroporation, microinjection, or PEG-mediated transfection. This kit utilizes an optimized PEG transfection method, leveraging the transient impact of PEG on protoplasts to facilitate the entry of plasmid DNA, RNA, or proteins into the protoplasts. It offers advantages such as convenience of use and high transfection efficiency.

When used in conjunction with Plant Protoplasts Isolation Kit, this kit can accomplish short-term or long-term expression of exogenous genes in model organisms like Arabidopsis protoplasts, or modulation (such as closure, knockdown, or editing) of endogenous genes.

Components

• Transfection Reagent
• Solution A
• Solution B
• Stop Solution
• Culture Medium

Storage

Store at -20°C; effective for one year. Transfection reagents can be stored at room temperature or 4°C. For multiple uses within 1-2 weeks, store at 4°C.

Precaution

• Store the relevant reagents according to the storage conditions specified in the kit. Thaw and mix each solution thoroughly before use.
• Sterile procedures must be followed during the use of this kit to avoid microbial contamination. Culturing media can be aliquoted and stored appropriately.
• This product is intended for scientific research by professionals only and should not be used for clinical diagnosis or treatment, food, or drugs, nor stored in residential homes.
• For your safety and health, wear laboratory attire and disposable gloves during operation.

Transfection Reagent Portfolio

• TransEZPEI™ Transfection Reagent
• TransEZ293F™ Transfection Reagent
• TransEZ293™ Transfection Reagent
• TransEZ6000™ Transfection Reagent
• TransEZ8000™ Transfection Reagent
• TransEZRNAi™ Transfection Reagent
• TransEZInsect™ Transfection Reagent

SBS Genetech is recognized as one of the global major leading industry players in Transfection Reagent by third-party market researchers. For more details, please visit Global Transfection Reagent and Equipment Market – Industry Trends and Forecast to 2030.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory