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Cat. No.: PPICD-2 (for 2ml)

Cat. No.: PPICD-10 (for 10ml)

Description

Phosphatase Inhibitor Cocktail D (50X) is a mixture of phosphatase inhibitors used for protein extraction from animal cells or tissues, fungi, bacteria, and plants, as well as for other related purposes. It contains 2.5mM (-)-p-bromotetramisole oxalate, 500μM Cantharidin, and 1μM Calyculin A in DMSO, and can broadly inhibit alkaline and Ser/Thr phosphatases in protein extracts.

Extracts from cells or tissues contain many endogenous proteases and phosphatases that can lead to protein degradation or dephosphorylation, affecting subsequent protein detection. Adding appropriate protease and phosphatase inhibitors to the extracts is an effective method to prevent protein degradation and dephosphorylation. This phosphatase inhibitor cocktail can effectively inhibit common phosphatases from dephosphorylating proteins, maintaining the original phosphorylation state of the proteins.

This product is an optimized and tested phosphatase inhibitor cocktail for protein extraction from animal cells or tissues, fungi, bacteria, and plants, containing inhibitors for alkaline and Ser/Thr phosphatases.

The phosphatase inhibitor cocktail contains 2.5mM (-)-p-bromotetramisole oxalate, 500μM Cantharidin, and 1μM Calyculin A, prepared in DMSO with a small amount of water.

This product is convenient to use; typically, it is added to the lysis buffer at a 1:50 ratio for protein extraction from samples, effectively inhibiting protein dephosphorylation.

This product can effectively inhibit alkaline and Ser/Thr phosphatases in protein extracts from animal tissues. (-)-p-bromotetramisole oxalate is an irreversible inhibitor of alkaline phosphatases; Cantharidin is a reversible inhibitor of protein phosphatase 2A with an IC50 of 40nM; and Calyculin A is a reversible inhibitor of Ser/Thr protein phosphatase 1 and protein phosphatase 2A.

Storage

Store at -20°C, valid for one year.

Precautions

• This product can be aliquoted and stored as needed, and should be added to lysis buffer or other appropriate solutions just before use.
• The phosphatase inhibitors in this product are harmful to humans. Handle with care and use effective protection to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or drugs, and should not be stored in ordinary residences.
• For your safety and health, please wear a lab coat and disposable gloves when handling.

Only for research and not intended for treatment of humans or animals

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