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phi29 HT DNA Polymerase
$375.00 - $2,000.00
phi29 HT DNA polymerase is an updated version of phi29 DNA polymerase. In addition to the strong strand displacement and continuous synthesis (> 70kb) activity of phi29 DNA polymerase, phi29 HT DNA polymerase can continuously synthesize DNA at 42°C, while the activity of phi29 DNA polymerase is very low at this temperature. In addition, phi29 HT DNA Polymerase still has a strong 3 '- 5' exonuclease proofreading function and the fidelity of the synthesized DNA fragments is high. The exonuclease activity of this enzyme is strong, so the primer needs 3'- end thio-modification in the process of synthesis to reduce the cleavage effect of the exonuclease activity on the primer.
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All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: P29PH-1k (for 1KU)
Cat. No.: P29PH-10k (for 10KU)
We also offer phi29 HT DNA Polymerase in lyophilized form, please contact us for more details.
Content
1KU
- phi29 HT DNA Polymerase (10 U/μl): 100 μl
- 10 × phi29 Buffer: 1 ml
10KU
- phi29 HT DNA Polymerase (10 U/μl): 1 ml
- 10 × phi29 Buffer: 1 ml × 3
Features
- Strong strand displacement capability
- Continuous synthesis capability
- High fidelity of amplification
Applications
- DNA isothermal rolling loop amplification
- DNA synthesis requiring strong strand displacement
- Rapid replication of plasmids
- Rapid replication requiring high fidelity
Advantages
The high-temperature reaction characteristic of phi29 HT DNA Polymerase has the following advantages:
- In the next generation sequencing (NGS), the enzyme has stronger amplification activity for complex templates such as high GC content and palindrome structure, which makes the coverage of NGS more uniform and reduces the depth required for sequencing.
- High-temperature reaction conditions improve the synthesis of WGA products of genomic DNA and can be used for variable temperature amplification.
- The gap region in sequencing is reduced, which can improve the quality and integrity of the data from single-cell sequencing.
- Reduce non-specific amplification products.
- Improve the amplification performance and specificity of MDA/RCA and other experiments.
Storage
The minimum shelf life is 3 years at -20°C.
Instruction: Protocol
Learn more: Choosing Your Best Phi29 DNA Polymerase and Padlock Primers to Start Your Rolling Circle Amplification
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Only for research and not intended for treatment of humans or animals