All products have special prices for bulk purchase, please contact for more details if required.

Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

We also offer DNA-free Pfu DNA Polymerase, which has undergone a rigorous E. coli DNA removal process.

Cat. No.: PFU-250 (for 250U, sample)

Cat. No.: PFU-200k (for 200KU)

The lyophilized Pfu DNA Polymerase is also available by inquiry, which can be transported at room temperature.

Description

Pfu DNA polymerase is isolated and purified from E. coli, which carries the cloned gene of Pyrococcus furiosus DNA polymerase. This enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high fidelity.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C.

Components

250U

• Pfu DNA Polymerase Storage Buffer: 20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, Stabilizers, 50% glycerol.
• 10 x Pfu Buffer: 200 mM Tris-HCl (pH8.8), 100 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgSO₄, etc.

Instruction: Protocol

Related: U-Taq DNA Polymerase, 2 x Pfu PCR MasterMix, sPfu DNA Polymerase, SuperGold™ 1×sPfu MasterMix

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Featured Citations

Interested in seeing published research using our Pfu DNA Polymerase?

New insights into the prevalence and phylogenetic diversity of Cysticercus ovis isolates in sheep from Sulaymaniyah

_{Journal of Veterinary Research    |    22 Jul 2021    |    DOI: https://doi.org/10.2478/jvetres-2021-0039}

_{The mitochondrial DNA was amplified using f-Pfu DNA polymerase according to the manufacturer’s protocol (SBS Genetech Co., Beijing, China), under the conditions previously described (23). The PCR products were confirmed via gel electrophoresis on 1.5% agarose gels (TBA, 0.5%) that were stained with GoodView Nucleic Acid Stain (SBS Genetech Co.).}

Molecular study of Cysticercus tenuicollis from slaughtered sheep in Sulaymaniyah province

_{Journal of Veterinary Research    |    12 May 2020    |    DOI: https://doi.org/10.2478/jvetres-2020-0034}

_{The DNA amplification was performed using f-Pfu DNA polymerase (SBS Genetech Co., Beijing, China) in a thermal cycler (Techne, Stone, U.K) under the conditions as described previously by Rostami et al. (18). The amplicons were assessed by electrophoresis in 1.5% (w/v) agarose gels using GoodView Nucleic Acid Stain (SBS Genetech Co.)}

Novel isolate of Cysticercus tenuicollis-Kalar isolate has been revealed in Kalar

_{Journal of Infection in Developing Countries    |    31 Oct 2021    |    DOI: https://doi.org/10.3855/jidc.12758}

_{The DNA amplification was performed by using f-Pfu DNA Polymerase (SBS Genetech Co., Beijing, China) under the conditions in the thermal cycler (Techne, U.K) as previously described by [10]. The amplicons were verified by gel electrophoresis on 1.5% agarose gels (TBA, 0.5%) stained with GoodViewTM Nucleic Acid Stain (SBS Genetech Co., Beijing, China).}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory