All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: PFDLGF-2k (for 2KU)

Cat. No.: PFDLGF-10k (for 10KU)

Description

Pfu DNA Ligase is derived from thermophilic microorganisms and utilizes ATP as a coenzyme. It catalyzes the ligation of adjacent 5'-phosphate and 3'-hydroxyl ends of double-stranded DNA within the temperature range of 45°C to 80°C. Due to its high thermal stability, it allows for higher reaction temperatures, thereby improving the fidelity of Ligase Chain Reaction (LCR). Pfu DNA Ligase exhibits higher ligation specificity compared to Taq DNA Ligase, making it particularly suitable for detecting single nucleotide polymorphisms (SNPs), such as in pharmacogenomics for gene typing to guide drug therapy. 

Our Pfu DNA Ligase (glycerol-free) is a recombinant protein expressed and purified through multiple steps, and it is glycerol-free.

Activity Definition

One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 μg of λ DNA fragments (with 12 bp sticky ends, approximately equivalent to 0.0338 pmole) digested with BstEII within 15 minutes in a 20 μl reaction system at 45°C.

Activity Assay Conditions

The assay is conducted at 45°C for 15 minutes in a reaction mixture containing 20 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 1 mM DTT, 1 mM ATP, 0.1% Tween 20, and 0.1 mg/ml BSA.

Features

• Excellent thermal stability, compatible with template denaturation in Ligase Chain Reaction (LCR).
• Extremely high fidelity; mispaired base pairs at gapped positions will not be ligated.
• Capable of ligating at cut sites on DNA strands under high-temperature conditions.
• Higher ligation specificity and lower non-specific background compared to room-temperature DNA ligases.
• Higher ligation specificity and lower non-specific background compared to Taq DNA Ligase.
• Reaction temperature range of 40-70°C, with increased specificity and activity at higher temperatures, approaching 80°C.

Applications

• Detection of allele-specific genes through Ligase Detection Reaction and Ligase Chain Reaction (LCR).
• Ligation of phosphorylated oligonucleotides in Ligase Chain Reaction (LCR).
• Ligation at cut sites on DNA strands under high-temperature conditions.

Storage

Store at -20°C.

DNA Ligase Products:

• T4 DNA Ligase
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032