Mutant Proteinase K (Sterile)
$1,000.00 - $100,000.00
Our Mutant Proteinase K is included in New Products, Science Journal, March 8, 2019.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: MPTKS-10 (for 10g)
Cat. No.: MPTKS-1000 (for 1kg)
Description
Mutant Proteinase K is derived from a genetically optimized strain of Kluyveromyces lactis expressing Engyodontium album proteinase K. It offers advantages such as higher specific activity, increased yield, and a broader range of temperature/pH activity compared to the wild-type enzyme. Advanced protein engineering techniques and up to 200,000-liter fermentation platform effectively reduce the unit cost, providing the most cost-effective proteinase K for biopharmaceutical research and production. Yeast high-level expression and chromatographic purification ensure product purity, free from bacterial endotoxins, and with a wider application range than natural proteinase K. Mutant Proteinase K is the most active among existing proteinases, with a pH activity range of 4-12 and a temperature range of 0-75°C, maintaining activity even in the presence of SDS, urea, or EDTA. It cleaves peptide bonds at the carboxyl end of both aliphatic and aromatic amino acids, enabling the degradation of all proteins.
Basic Information:
Attribute | Details |
Product Grade | Molecular biology grade, non-sterile powder |
Catalog Number | MPTKS |
CAS Number | 39450-01-6 |
E.C. | 3.4.21.64 |
Product Size | 100g/bottle, etc. |
Molecular Weight | 29.3KD |
Shelf Life | Effective for 3 years when stored in a dry environment below 4°C after receipt |
Uses | - Used in gene diagnostic kits, genome DNA extraction kits, and RNA extraction kits to remove nucleases and other protein contaminants. - Extracting nucleic acids, polysaccharides, and other non-protein biological products in the biopharmaceutical industry, such as DNA vaccines and heparin preparation. - Used in skincare to remove aging keratinocytes, tanning, preparation of hydrolyzed protein flavorings, cosmetics, and processing of feed, and other daily industrial fields. |
Instructions | - Prepare a solution of proteinase K solid at 20-40mg/ml. - Add proteinase K to a final concentration of 50-200µg/ml in the digestion or lysis solution. - The duration of action varies from 30 minutes to 18 hours depending on the desired effect. Prolonged reactions may result in self-degradation of proteinase K. |
PI | 8.9 |
Storage Conditions | Can be transported and stored at room temperature for short periods. It is recommended to store sealed at a temperature below 4°C. |
Storage Buffer | 20mM Tris-HCl (pH 7.4), 1mM CaCl₂, 50% glycerol |
Dilution Buffer | - 20mM Tris-HCl (pH 7.4), 1mM CaCl₂. - Or 20mM Tris-HCl (pH 7.4), 1mM CaCl₂, 2% glycerol. |
Source Species | Kluyveromyces lactis expressing genetically engineered Engyodontium album proteinase K gene |
QC Assays
Attribute | Details |
Appearance | White or off-white lyophilized powder |
Enzyme Activity | ≥30U/mg lyophilized powder |
Optimal pH Range | 7.5-11.5 |
Optimal Temperature Range | Recommended temperature range is 37-70°C, with maximum activity at 70°C |
Inactivation Method | Addition of 5mM PMSF to the reaction system |
Inhibitors | Diisopropyl fluorophosphate (DIFP), Phenylmethylsulfonyl fluoride (PMSF) |
Unit Activity Definition | One unit (U) of proteinase K is defined as the amount that hydrolyzes casein substrate to produce 1µmol of tyrosine per minute at pH 7.5 and 37°C. Enzyme activity values for each batch are provided in the corresponding quality inspection report. |
Residual Impurities | - Does not contain DNA and RNA, DNase and RNase free. - No detectable deoxyribonuclease activity at 37°C for over 3 hours using λDNA as a substrate. - No detectable ribonuclease activity at 37°C for over 3 hours using RNA as a substrate. |
Electrophoretic Purity | ≥95% (Native-PAGE) |
Activators | 1-5mM Ca²⁺ |
Procedure for preparing sterile solution of 20mg/ml-40mg/ml Mutant Proteinase K
- Use a filter with a pore size of 0.22 µm to sterile-filter the buffer [20mM Tris-HCl (pH 7.4), 1mM CaCl₂, 50% glycerol].
- Add the filtered buffer to the sterile Mutant Proteinase K lyophilized powder.
Notes:
a. This molecular biology grade Mutant Proteinase K lyophilized powder is a sterile product. Steps 1 and 2 above must be carried out in a sterile environment.
b. Buffer: [20mM Tris-HCl (pH 7.4), 1mM CaCl₂, 50% glycerol]. This concentration refers to the final concentration in the solution volume.
c. The sterile Mutant Proteinase K solution can be stored at temperatures ranging from 24°C to -80°C to maintain enzyme stability. If the Mutant Proteinase K solution needs to be used repeatedly, it should be aliquoted and stored.
Storage
Short-term transportation and storage at room temperature is acceptable. It is recommended to store the product sealed and at a temperature below 4°C.
References:
- Kraus, E; et.al. Proteinase K from the Mold Tritirachium album limber, Specificity and Mode of Action. Z. Physiol. Chem., 357:939;1976.
- Jany,KD, et al. Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album limber. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letter, 199,139.1986.
Mutant Proteinase K is included in New Products, Science Journal, March 8, 2019. Please visit:
SBS Genetech is recognized as one of the global major leading industry players in Proteinase K by third-party market researchers. For more details, please visit Proteinase K Market Share, Size, Trends, Industry Analysis Report, By Therapeutic Area; By Form, By Regions; Segment Forecast, 2020-2027.
Related:
- Mutant Proteinase K: for common molecular biology applications
- Mutant Proteinase K (Sterile): for high-standard applications
- Mutant Proteinase K (Industrial Grade): for industrial applications
Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.
Only for research and not intended for treatment of humans or animals
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