Mutant Proteinase K (Industrial Grade)
$20.00 - $1,000.00
Our Mutant Proteinase K is included in New Products, Science Journal, March 8, 2019.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: MPTKI-10 (for 10g)
Cat. No.: MPTKI-1000 (for 1kg)
Description
Proteinase K, also known as keratinase, is derived from microorganisms that digest hair and keratin. This product is engineered through protein engineering, with the gene originating from Engyodontium album, a strain of Rhizopus that has been optimized through site-directed mutagenesis. The gene is then expressed and purified through recombinant yeast expression.
Our Mutant Proteinase K (Industrial Grade) boasts higher specific activity and a broader temperature range (4-85°C) as well as a wider pH activity range (pH 4-12). Advanced protein engineering techniques combined with large-scale yeast cultivation platforms effectively reduce the unit cost, making it the most cost-effective proteinase K option for biopharmaceutical research and production enterprises.
Our Mutant Proteinase K is among the most active proteinases available, with an optimal pH range of 4-12 and a temperature range of 4-85°C. Its activity remains unaffected in the presence of SDS, urea, or EDTA. Its mechanism involves cleaving peptide bonds at the carboxyl end of both aliphatic and aromatic amino acids, enabling the degradation of all proteins into peptides and amino acids. It can effectively replace other protease family products such as alkaline proteases, acidic proteases, neutral proteases, papain, and bromelain, simplifying industrial processes and reducing production costs.
Our company primarily produces molecular biology-grade Mutant Proteinase K, commonly used in gene diagnostic kits, genome DNA extraction kits, and RNA extraction kits to remove nucleases and other protein contaminants.
Basic Information
Attribute | Details |
Product Grade | Industrial grade |
Catalog Number | MPTKI |
CAS Number | 39450-01-6 |
E.C. | 3.4.21.64 |
Product Size | 10g/bottle, 50g/bottle |
Molecular Weight | 29.3KD |
Dilution Buffer | Distilled water or buffer solution (pH neutral to alkaline) |
Shelf Life | Effective for 3 years when stored in a dry environment below 4°C after receipt |
Uses | - Used in the biopharmaceutical industry to extract nucleic acids, polysaccharides, and other non-protein biological products, such as DNA vaccines and heparin preparation, to remove impurities. - Also used in leather tanning, preparation of hydrolyzed protein flavorings, laundry detergents, and feed processing in daily industrial fields. |
PI | 8.9 |
Source Species | Derived from Kluyveromyces lactis expressing genetically engineered Engyodontium album proteinase K gene |
QC Assays
Attribute | Details |
Appearance | Light yellow lyophilized powder |
Enzyme Activity | ≥150U/g lyophilized powder |
Optimal pH Range | 7.5-11.5 |
Optimal Temperature Range | Recommended temperature range is 37-70°C, with maximum activity at 70°C |
Inactivation Method | Addition of 5mM PMSF to the reaction system |
Inhibitors | Diisopropyl fluorophosphate (DIFP), Phenylmethylsulfonyl fluoride (PMSF) |
Unit Activity Definition | One unit (U) of Proteinase K is defined as the amount that hydrolyzes casein substrate to produce 1µmol of tyrosine per minute at pH 7.5 and 37°C. Enzyme activity values for each batch are provided in the corresponding quality inspection report. |
Activators | 1-5mM Ca²⁺ |
Storage
Short-term transportation and storage at room temperature is acceptable. It is recommended to store the product sealed and at a temperature below 4°C.
References:
- Kraus, E; et.al. Proteinase K from the Mold Tritirachium album limber, Specificity and Mode of Action. Z. Physiol. Chem., 357:939;1976.
- Jany,KD, et al. Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album limber. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letter, 199,139.1986.
Mutant Proteinase K is included in New Products, Science Journal, March 8, 2019. Please visit:
SBS Genetech is recognized as one of the global major leading industry players in Proteinase K by third-party market researchers. For more details, please visit Proteinase K Market Share, Size, Trends, Industry Analysis Report, By Therapeutic Area; By Form, By Regions; Segment Forecast, 2020-2027.
Related:
- Mutant Proteinase K: for common molecular biology applications
- Mutant Proteinase K (Sterile): for high-standard applications
- Mutant Proteinase K (Industrial Grade): for industrial applications
Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.
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