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Description

qPCR (Quantitative PCR), also known as real-time quantitative PCR or real-time PCR, is a method for quantifying DNA by measuring fluorescence during each cycle of the PCR process. This technique uses two common methods: the SYBR Green method and the probe method.

The SYBR Green method employs a non-specific fluorescent DNA-binding dye, like SYBR Green, to detect the accumulation of PCR products during the process. The probe method, often referred to as the TaqMan probe method, uses DNA probes labeled with a fluorophore and a quencher to target specific sequences for detection.

For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.

The product is provided as a pre-mixed lyophilized powder, with each tube containing 1 nmol of forward and reverse primers (2 nmol in total), nuclease-free. Simply add 400 μl of ultra-pure water to dissolve to a concentration of 2.5 μM each. Use 2 μl of primer in a 20 μl or 25 μl reaction system, allowing each tube to be used for 200 qPCR experiments.

Storage

Store at -20°C. It is recommended to aliquot after reconstitution to avoid repeated freeze-thaw cycles.